Evaluation of the Physicochemical Characteristics of Eucalyptus Oil from Two Distillation Methods

Original Article

Authors

DOI:

https://doi.org/10.33936/riemat.v9i2.7043

Keywords:

Essential oil; steam entrainment; physicochemical characteristics; extraction; hydrodistillation; yield.

Abstract

Steam extraction and hydrodistillation are still interesting to researchers who want to extract essential oils. This study aimed to evaluate the physicochemical characteristics of eucalyptus essential oil. The extraction was carried out by steam drag and hydrodistillation for 122 and 140 minutes. The refractive index, density, solubility, acidity, color, iodine value, fatty acids, and yield were determined for the oil obtained. The results indicated that hydrodistillation (HD), with an extraction time of 140 minutes, yielded a percentage greater than 40% compared to the other treatments. There were no discernible differences in iodine value and refraction statistics. The relative density exhibited higher values in the vapor entrainment (AV) treatment and the control compared to the rest. The acidity level was higher in the control group, possibly due to a higher presence of saturated free fatty acids caused by inadequate storage or exposure to light. Fatty acid profile analysis revealed that the total unsaturated content was highest in the commercial oil, followed by hydrodistillation of monounsaturated and vapor carryover of polyunsaturated. It was concluded that research should continue to be carried out on the HD method compared to other newer ones to verify its effectiveness.

Downloads

Download data is not yet available.

References

Abbas, A., Anwar, F., Alqahtani, S., Ahmad, N., Al-Mijalli, S., Shahid, M., and Iqbal, M. (2022). Hydro-Distilled and Supercritical Fluid Extraction of Eucalyptus camaldulensis Essential Oil: Characterization of Bioactives Along with Antioxidant, Antimicrobial and Antibiofilm Activities. Dose-Response, 20(3), 1–12. https://doi.org/10.1177/15593258221125477

Ahmad, R. S., Imran, M., Ahmad, M. H., Khan, M. K., Yasmin, A., Saima, H., Abbas, K., Chaudhary, R., and Rahim, M. A. (2023). Eucalyptus essential oils (G. A. Nayik & M. J. B. T.-E. O. Ansari (eds.); pp. 217–239). In Essential Oils (pp. 217-239). Academic Press. https://doi.org/10.1016/B978-0-323-91740-7.00005-0

AOAC 920.212. (1995). Specific gravity. (Apparent) of Oils, Pycnometer method. Association of Official Analytical Chemist, Washington DC.

AOCS Official Method Cc 13e-92. (2017). Commercial fats and oils. Color of Fats and Oil, Lovibond (ISO Method). https://pdfcoffee.com/qdownload/aocs-cc-13e-92-color-2017-3-pdf-free.html

AOCS Official Method Cd-1d-92. (2009). Sampling and analysis of commercial fats and oils. Iodine Value of Fats and Oils, Cyclohexane-Acetic Acid Method. Official Methods and Recommended Practices of the AOCS. https://pdfcoffee.com/qdownload/aocs-cd-1d-92-pdf-free.html.

AOCS Ce 1h-05. (2005). Determination of cis-, trans-, saturated, monounsaturated and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC. Official Methods and Recommended Practices of the AOCS, Sixth edition. https://www.aocs.org/attain-lab-services/methods/methods/search-results?method=111777

Boukhatem, M. N., Amine, F. M., Kameli, A., Saidi, F., Walid, K., and Mohamed, S. M. (2014). Quality assessment of the essential oil from Eucalyptus globulus Labill of Blida (Algeria) origin. International Letters of Chemistry, Physics and Astronomy, 17(3), 303–315. https://doi.org/10.18052/www.scipress.com/ILCPA.36.303

Calderón, M., y Loor, M. (2023). Influencia del tiempo y contenido de humedad en el rendimiento del aceite esencial obtenido de hojas de eucalipto frescas y liofilizadas [Tesis de grado, Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López]. https://repositorio.espam.edu.ec/bitstream/42000/2059/1/TIC_AI21D.pdf

Cedeño, A., Moreira, C., Muñoz, J., Muñoz, A., Pillasaguay, S., y Riera, M. (2019). Comparación de métodos de destilación para la obtención de aceite esencial de eucalipto. Revista Colón Ciencias, Tecnología y Negocios, 6(1), 1-13. https://revistas.up.ac.pa/index.php/revista_colon_ctn/article/view/472/383

Cisneros Hilario, C. B. (2022). Mejora del proceso de destilación por arrastre con vapor de agua para incrementar el nivel de producción de aceites esenciales. Chimbote – 2022. [Tesis de grado, Universidad César Vallejo]. https://repositorio.ucv.edu.pe/bitstream/handle/20.500.12692/114836/Cisnero_HCB-SD.pdf?sequence=1&isAllowed=y

Colina-Márquez-Márquez, J. Ángel., Contreras, E., Ruiz, J., y Monroy, L. (2022). Comparación de dos métodos de extracción para el aceite esencial de la cáscara de pomelo (Citrus maxima). Revista Ing-Nova, 1(1), 85–98. https://doi.org/10.32997/rin-2022-3729

Ebadollahi, A., Ziaee, M., and Palla, F. (2020). Essential oils extracted from different species of the Lamiaceae plant family as prospective bioagents against several detrimental pests. Molecules, 25(7), 1–15. https://doi.org/10.3390/molecules25071556

Edah, A., Ede, R., Stanley, V., and Samuel, J. (2019). Physicochemical Properties and Antimicrobial activities of Essential oil extracted from Eucalyptus globulus leaves. Physicochemical. International Journal of Chemical Science, 3(5), 1–4. https://www.chemicaljournals.com/assets/archives/2019/vol3issue5/3-4-32-899.pdf

Farinango Guzmán, W. D. (2021). Diseño de un proceso industrial para obtener aceite esencial de Eucalipto (Eucalyptus globulus labill) en la empresa IFG innovaciones agropecuarias Ibarra-Ecuador. http://dspace.espoch.edu.ec/handle/123456789/16743

Granados, C., Santafé, G., y Acevedo, D. (2015). Composición química y evaluación de la actividad antioxidante del aceite esencial foliar de Eucalyptus camaldulensis de Norte de Santander (Colombia). Revista U.D.C.A Actualidad & Divulgación Científica, 18(1), 235–240. https://doi.org/10.31910/rudca.v18.n1.2015.477

Gutiérrez-Jiménez, E., Pedroza-Sandoval, A., Martínez-Bolaños, L., Samaniego-Gaxiola, J. A. y García-González, F. (2018). Efecto de aceites naturales como antimicrobiano en condiciones in vitro y detección de fitoquímicos activos. Revista Mexicana de Fitopatología, 36(1), 141–150. https://doi.org/10.18781/r.mex.fit.1707-4

Ikbal, C., and Pavela, R. (2019). Essential oils as active ingredients of botanical insecticides against aphids. Journal of Pest Science, 92(3), 971–986. https://doi.org/10.1007/s10340-019-01089-6

Immaroh, N., Kuliahsari, D., and Nugraheni, S. (2021). Review: Eucalyptus globulus essential oil extraction method. International Conference on Green Agro-industry and Bieconomy, 733. https://doi.org/10.1088/1755-1315/733/1/012103

Kiferle, C., Ascrizzi, R., Martinelli, M., Gonzali, S., Mariotti, L., Pistelli, L., Flamini, G., and Perata, P. (2019). Effect of Iodine treatments on Ocimum basilicum L.: Biofortification, phenolics production and essential oil composition. PLoS ONE, 14(12), e0226559. https://doi.org/10.1371/journal.pone.0226559

López De La Cruz, R. P., y Caso Orihuela, N. V. (2015). Rendimiento y composición química de aceites esenciales de Eucalyptus archeri y Schinus molle [Tesis de grado, Universidad Nacional del Centro del Perú]. https://repositorio.uncp.edu.pe/handle/20.500.12894/3508

Ministerio de agricultura y Ganadería (MAG). (2017). El Eucalipto genera impactos sobre la naturaleza. El Comercio. https://www.elcomercio.com/tendencias/sociedad/eucalipto-impactos-naturaleza-incendios-fuego.html

Moreno, J., López, G., and Siche, R. (2016). Modeling and optimization of extraction process of eucalyptus essential oil (Eucalyptus globulus). Scientia Agropecuaria, 1(2), 147–154. https://doi.org/10.17268/sci.agropecu.2010.02.05

Ngo, T. C. Q., Tran, T., H., and Le, X. T. (2020). The effects of influencing parameters on the Eucalyptus globulus leaves essential oil extraction by hydrodistillation method. IOP Conference Series: Materials Science and Engineering, 991(1), 12126. https://doi.org/10.1088/1757-899X/991/1/012126

Nolazco Cama D., Villanueva-Quejia, E., Hatta Sakoda, B., y Tellez Monzon, L. (2020). Extracción y caracterización química del aceite esencial de Eucalipto obtenido por microondas y ultrasonido. Revista de Investigaciones Altoandinas, 22(3), 274–284. https://dx.doi.org/10.18271/ria.2020.661

Oliveira, C. S. D., Moreira, P., Cruz, M. T., Pereira, C. M. F., Silva, A. M. S., Santos, S. A. O., & Silvestre, A. J. D. (2023). Exploiting the Integrated Valorization of Eucalyptus globulus Leaves: Chemical Composition and Biological Potential of the Lipophilic Fraction before and after Hydrodistillation. In International Journal of Molecular Sciences (Vol. 24, Issue 7). https://doi.org/10.3390/ijms24076226

Quispe Solano, M. A. Corilla Flores, D. D., Asto Hinojosa, R., De La Cruz A. H. y Manyari Cervantes, G. M. (2022). Capacidad antioxidante de aceite esencial de hojas de eucalipto (Eucalyptus globulus) extraído por energía ultrasónica. Ciencia agroalimentaria, 1(1), 19–29. https://revistas.uncp.edu.pe/index.php/jafs/article/view/1437/1605

Romero, M. (2018). Usos tradicionales y actuales de los aceites esenciales. [Tesis de grado, Universidad de Sevilla]. https://idus.us.es/bitstream/handle/11441/82290/TFG%20-%20Maria%20Romero%20Alcedo.pdf;jsessionid=9FDB4520FD6E3F03CA7980FFB546A5C1?sequence=1&isAllowed=y

Sarracent-López, A., y Gandón-Hernández, J. (2016). Estudio de la transformación del aceite de soya usado en ésteres etílicos de ácidos grasos. Tecnología Química, 36(3), 417–438. http://scielo.sld.cu/scielo.php?pid=S2224-61852016000300009&script=sci_arttext

Sharma, A., Kumar, V., Mittal, C., Rana, D. V., Dabral, K., and Parveen, G. (2023). Role of essential oil used pharmaceutical cosmetic product. Journal for Research in Applied Sciences and Biotechnology, 2(3), 147–157. https://doi.org/10.55544/jrasb.2.3.19

Torrenegra, A. M. E., Granados, C. C., y León, M. G. (2019). Extracción, caracterización y actividad antioxidante del aceite esencial de Eucalyptus globulus Labill. Revista Cubana de Farmacia, 52(1), 1–12. https://www.medigraphic.com/pdfs/revcubfar/rcf-2019/rcf191g.pdf

Published

2024-10-15