Evaluation of Bacillus amyloliquefaciens in the control of Botrytis cinerea in Rosa sp.

Authors

DOI:

https://doi.org/10.33936/latecnica.v13i1.5542

Keywords:

bacteria; biological control; mycology; plant pathology.

Abstract

In the first two months of 2022, flower exports reached 197 million dollars, representing a 7% increase compared to the same period in 2021. To maintain this growth rate, it is important to efficiently control pathogens that threaten productivity, such as Botrytis cinerea, which causes significant crop loss in roses. Therefore, the objective of this research was to evaluate the effectiveness of Bacillus amyloliquefaciens in controlling B. cinerea in Rosa sp. A completely randomized design with four treatments and three replications was used to compare the frequency of application of B. amyloliquefaciens versus chemical management. Treatment application of B. amyloliquefaciens every 4 days (A2) maintained an incidence and severity percentage between 0 and 2%, while treatment A1 ranged between 10 and 14%. Regarding the variable vase life, treatment A2 achieved 83% of roses in good condition, while treatment A1 reached 63%. These results clearly demonstrate the effectiveness of using biological products.

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References

Agrios, G. N. (2005). Plant pathology. Elsevier. In: Elsevier Inc. all rights reserved. https://doi.org/10.1242/ jcs.207373

Ambrico, A., y Trupo, M. (2017). Efficacy of cell free supernatant from Bacillus subtilis ET-1, an Iturin A producer strain, on biocontrol of green and gray mold. Postharvest Biology and Technology, 134, 5-10. https://doi.org/10.1016/j.postharvbio.2017.08.001

Arrebola, E., Sivakumar, D., Bacigalupo, R. and Korsten, L. (2010). Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases. Crop Protection, 29(4), 369-377. https://doi.org/10.1016/j.cropro.2009.08.001

Badii, M. H., Castillo, J., Rodríguez, M., Wong, A., y Villalpando, P. (2007). Diseños experimentales e investigación científica. Revista Innovaciones de Negocios, 4(2), 283-330. https://doi.org/10.29105/rinn4.8-5

Calvo-Garrido, C., Roudet, J., Aveline, N., Davidou, L., Dupin, S. and Fermaud, M. (2019). Microbial antagonism toward Botrytis bunch rot of grapes in multiple field tests using one Bacillus ginsengihumi strain and formulated biological control products. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00105

CERTIS agricultura sostenible y rentable. (2021). AMYLO-X® WG. Fungicida-bactericida de origen natural, I(1), 3. España. https://www.certiseurope.es/fileadmin/ES/Descargas/Productos/Solution_Finder/AMYLO-X_WG/Folleto_Amylo-X_WG_Lechuga.pdf

Fedele, G., Brischetto, C. and Rossi, V. (2020). Biocontrol of Botrytis cinerea on grape berries as influenced by temperature and humidity. Frontiers in Plant Science, 11, 1-11. https://doi.org/10.3389/fpls. 2020.01232

Gestiones y Representaciones Chía. (2019). Monitoreo directo plagas y enfermedades. Colombia.Gestiones y Reprensentaciones Chía. (2020). Procedimiento para la simulación de viaje y evaluación de vida en florero. Colombia.

Gotor-Vila, A., Teixidó, N., Casals, C., Torres, R., De Cal, A., Guijarro, B. and Usall, J. (2017). Biological control of brown rot in stone fruit using Bacillus amyloliquefaciensCPA-8 under field conditions. Crop Protection, 102, 72-80. https://doi.org/10.1016/j.cropro.2017.08.010

Hamdache, A., Ezziyyani, M. and Lamarti, A. (2018). Effect of preventive and simultaneous inoculations of Bacillus amyloliquefaciens (Fukumoto) strains on conidial germination of Botrytis cinerea Pers.:Fr. Anales de Biología, 40, 65-72. https://doi.org/10.6018/analesbio.40.08

Htwe-Maung, C. E., Seon-Baek, W., Gyu-Choi, T. and Yong-Kim, K. (2021). Control of grey mould disease on strawberry using the effective agent, Bacillus amyloliquefaciensY1. Biocontrol Science and Technology, 31(5), 468-482. https://doi.org/10.1080/09583157.2020.1867707

Jamal, Q., Lee, Y. S., Jeon, H. D., Park, Y. S. and Kim, K. Y. (2015). Isolation and biocontrol potential of Bacillus amyloliquefaciens Y1 against fungal plant pathogens. Korean Journal of Soil Science and Fertilizer, 48(5), 485-491. https://doi.org/10.7745/kjssf.2015.48.5.485

Le, U. T. (2022). Isolation and identification of the antagonistic bacteria against Xanthomonas spp. causing the leaf spot from Rosa spp. Can Tho University Journal of Science, 14, 68-73. https://doi.org/10.22 144/ctu.jen.2022.030

Liu, X., Cao, X., Shi, S., Zhao, N., Li, D., Fang, P., Chen, X., Qi, W. and Zhang, Z. (2018). Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection. BMC Genetics, 19(1), 1-10. https://doi.org/10.1186/s12863-018-0668-x

Mari, M., Guizzardi, M., Brunelli, M. and Folchi, A. (1996). Postharvest biological control of grey mould (Botrytis cinerea pers.: Fr.) on fresh-market tomatoes with Bacillus amyloliquefaciens.Crop Protection, 15(8), 699-705. https://doi.org/10.1016/s0261-2194(96)00042-7

Masmoudi, F., Ben Khedher, S., Kamoun, A., Zouari, N., Tounsi, S. and Trigui, M. (2017). Combinatorial effect of mutagenesis and medium component optimization on Bacillus amyloliquefaciens antifungal activity and efficacy in eradicating Botrytis cinerea. Microbiological Research, 197, 29-38. https://doi.org/10.1016/j.micres.2017.01.001

OpenAg®. OpenAgriculture. (2018). Producto biológico para el control de Botrytis. Biofungicida Amylo-X. Blanca Luz Pinilla. https://cl.uplonline.com/download_links/3yAT39PiptG8VSANk9u3Phuy WaWvBUQQYLpXm2UG.pdf

Pedraza-Herrera, L. A., Lopez-Carrascal, C. E. y Uribe-Vélez, D. (2020). Mecanismos de acción de Bacillus spp. (Bacillaceae) contra microorganismos fitopatógenos durante su interacción con plantas. Acta Biológica Colombiana, 25(1), 112-125. https://doi.org/10.15446/abc.v25n1.75045

Quinaluisa, C., Villamar, R., Díaz, E., Moncayo, O., López, J. and Jazeyeri, S. (2021). State of the art of floriculture in Ecuador: Historical and current economic context, genetic improvement and carbon footprint. Nexo Agropecuario, 9(1), 111-120. https://revistas.unc.edu.ar/index.php/nexoagro/ article/ view/32799

Rivera, T., y Cajan, J. (2016). Productos biológicos y químicos para el control de pudrición gris y ácida en el cultivo de vid (Vitis vinifera L.) en condiciones de campo, Chongoyape 2015. UCV-HACER. Revista de Investigación y Cultura, 5(2).

Rotolo, C., De Miccolis Angelini, R. M., Dongiovanni, C., Pollastro, S., Fumarola, G., Di Carolo, M., Perrelli, D., Natale, P. and Faretra, F. (2017). Use of biocontrol agents and botanicals in integrated management of Botrytis cinerea in table grape vineyards. Pest Management Science, 74(3), 715-725. https://doi.org/10.1002/ps.4767

Published

2023-06-28

How to Cite

Campués Cholca, S. V., Vásquez Hernández, L. del R., Basantes Vizcaíno, T. F., & Pabón Garcés, G. J. (2023). Evaluation of Bacillus amyloliquefaciens in the control of Botrytis cinerea in Rosa sp. La Técnica. Revista De Las Agrociencias. ISSN 2477-8982, 13(1), 37–46. https://doi.org/10.33936/latecnica.v13i1.5542

Issue

Section

Agricultura y Silvicultura