Seasonal dynamics and efficiency of capture systems for the isolation of native soil microorganisms in tropical conditions in El Carmen, Ecuador
Authors
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Leonardo Avellán-Vásquez
Universidad Laica Eloy Alfaro de Manabí
https://orcid.org/0000-0003-4265-8049
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Cesar Cruz-Rey
Investigadores independientes
https://orcid.org/0009-0004-9223-9314
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María Chango-Quishpe
Investigadores independientes
https://orcid.org/0009-0003-1584-5006
- Tamara Pinargote-Muñoz Universidad Laica Eloy Alfaro de Manabí
DOI:
https://doi.org/10.33936/la_tecnica.v16i1.8427Keywords:
microbiology, isolation, functional bacteria, dynamics, soils.Abstract
The aim of this study was to evaluate seasonal dynamics and the efficiency of different capture systems for the isolation of functional native microorganisms in tropical soil from El Carmen, Ecuador. A factorial design was implemented, integrating trap type, substrate type, locality, and season (dry and rainy). Phosphate-solubilizing microorganisms, nitrogen-fixing bacteria, actinomycetes, and cellulolytic microorganisms were quantified. The results showed that capture efficiency was significantly influenced by the interaction among experimental factors. For phosphate-solubilizing microorganisms, the “caneca–liquid substrate” combination reached the highest value (2.57 × 10³ CFU g⁻¹), whereas during the dry season the performance pattern varied according to the matrix used. Regarding actinomycetes, the tarrina-type trap consistently presented the highest counts, reaching up to 3.0 × 10⁸ CFU g-1, indicating greater microenvironmental stability of the system. For cellulolytic microorganisms, although the liquid substrate showed higher average values, no robust statistical differences were detected among most treatments. Furthermore, the rainy season recorded a higher overall mean (2.31 × 10⁶ CFU g⁻¹) compared to the dry season (1.69 × 10⁶ CFU g⁻¹), confirming the determining role of water availability in microbial activity. It is concluded that capture system architecture, its interaction with substrate type, and seasonality are key factors for optimizing the isolation of functional microorganisms in tropical soils, providing methodological foundations for microbial bioprospecting and the development of agricultural bioinputs.
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Copyright (c) 2026 Leonardo Avellán-Vásquez, Cesar Cruz-Rey, María Chango-Quishpe, Tamara Pinargote-Muñoz
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