Efecto de probióticos sobre el crecimiento de semillas de ostión del pacífico Crassostrea gigas

Effect of probiotics on the growth of oyster seeds from the pacific Crassostrea gigas

  • Milagro García Bernal Universidad Central de Las Villas, Cuba
  • Ricardo Medina Marrero Universidad Central de Las Villas, Cuba
  • Ángel Isidro Campa Córdoba Centro de Investigaciones Biológicas del Noroeste, México
  • José Delfino Barajas Frías Centro de Investigaciones Biológicas del Noroeste, México
  • Irasema Elizabeth Luis Villaseñor Universidad Autónoma de Sinaloa, México
  • José Manuel Mazón Suástegui Centro de Investigaciones Biológicas del Noroeste, México

Resumen

 
Se  realizó  un  estudio  en  juveniles  de  Crassostrea  gigas  para investigar el potencial efecto  probiótico de Streptomyces  spp. (cepas V4, N7 y RL8) y de una mezcla de Bacillus (BMix) teniendo como variable de respuesta su  crecimiento.  Las  bacterias  fueron  adicionadas  con  el  alimento  vivo (microalgas),  para  ser  ingeridas  por  los  ostiones  mediante  filtración.  El  diseño experimental  incluyó  cuatro  tratamientos  bacterianos:  [T1  (Streptomyces  sp. V4); T2 (Streptomyces sp. N7); T3 (Streptomyces sp. RL8); T4, una mezcla de bacilos  BMix  (Bacillus  tequilensis  YC5-2,  Bacillus  endophyticus  C2-2  y Bacillus  endophyticus  YC3-B]  y  un  tratamiento  Control  (T5)  con  solo microalgas  como  alimento.  Estas  cepas  potencialmente  probióticas  fueron suministradas a una concentración final de 1 × 106 UFC mL-1 y las microalgas (Isochrysis galbana y Chaetoceros calcitrans en proporción 1:1) a una densidad de 70-80 × 103 células mL-1). Los tratamientos T1 y T3 (Streptomyces spp. V4 y RL8,  respectivamente),  incrementaron  significativamente  el  área  y  el  diámetro teórico de la concha de C. gigas. Esto sugiere que la adición Strepromyces spp. V4  y  RL8  puede  mejorar  el  crecimiento  de  C.  gigas  durante  el  proceso  de preengorda de semillas en laboratorio. 
Palabras clave: Probióticos; Streptomyces;  Bacillus;  Crassostrea gigas 
 
Abstract
A  study  was  conducted  in  juveniles  of  Crassostrea  gigas  to investigate  the  potential  probiotic  effect  of  Streptomyces  spp.  (strains  V4,  N7 and  RL8)  and  a  mixture  of  Bacillus  (BMix)  having  as  a  response  variable  its growth. The bacteria were added with the live food (microalgae), to be ingested by  the  oysters  by  filtration.  The  experimental  design  included  four  bacterial treatments:  [T1  (Streptomyces  sp.  V4);  T2  (Streptomyces  sp.  N7);  T3 (Streptomyces  sp.  RL8);  T4,  a  mixture  of  BMix  bacilli  (Bacillus  tequilensis YC5-2,  Bacillus  endophyticus  C2-2  and  Bacillus  endophyticus  YC3-B)  and  a Control treatment (T5) with only microalgae as food. Those potential probiotic strains  were  supplied  at  a  final  concentration  of  1  ×  106  CFU  mL-1  and microalgae (Isochrysis galbana and Chaetoceros calcitrans in a 1: 1 ratio) at a density of 70-80 × 103 mL-1 cells). Treatments T1 and T3 (Streptomyces spp. V4 and  RL8,  respectively),  significantly  increased  the  area  and  the  theoretical diameter of the C. gigas shell. It suggest that the addition Strepromyces spp. V4 and RL8 can improve the growth of C. gigas during the process of spat nursery and hatchery seed production. 
Keywords: Probiotics; Streptomyces; Bacillus; Crassostrea gigas 

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Publicado
2020-05-13
Sección
Artículo Original