Presence of amyloid protein in the yellow clam Amarilladesma mactroides (Reeve, 1854), an emerging species for aquaculture
DOI:
https://doi.org/10.33936/at.v3i1.3439Palabras clave:
Almeja amarilla, Rojo Congo, Invertebrados, HistologíaResumen
Las proteínas amiloides son grupos proteicos que comprenden una superfamilia de apolipoproteínas altamente conservadas en vertebrados, estando implicadas en la modulación de numerosas respuestas inmunes durante infecciones, lesiones o estrés. En este estudio, la almeja amarilla Amarilladesma mactroides se utilizó como modelo para invertebrados y demostrar depósitos de proteína amiloide en sus tejidos. Se recolectaron de la playa ejemplares adultos de este bivalvo y se transportaron al Laboratorio de Inmunología y Patología de Organismos Acuáticos (LIPOA) de la Universidad Federal de Rio Grande (FURG). Sus tejidos se procesaron para su estudio histológico y se tiñeron con hematoxilina y eosina, y Rojo Congo. Las secciones histológicas teñidas con hematoxilina y eosina se examinaron con microscopía óptica clásica. Además, las secciones fueron teñidas con Rojo Congo y se analizaron con microscopía de luz polarizada. La tinción con Rojo Congo reveló la presencia de proteínas amiloides en el pie muscular y en las glándulas digestivas de la almeja amarilla. Las técnicas utilizadas demostraron ser adecuadas para la determinación de proteína amiloide en esta especie y evidenciaron un papel complementario de las proteínas amiloides en la respuesta inmune, indicando que pueden servir como marcador del sistema inmunológico innato y la salud de los invertebrados. La capacidad de evaluar la condición inmunológica puede proporcionar información relacionada con el cultivo de especies emergentes para la acuicultura.
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Carvalho Y.B.M., Santos J.J.S., Raibenberg F.R., Poersch L.H., Romano L.A. (2016). Use of polymerase chain reaction for bivalve pathogen surveillance in the yellow clam Mesodesma mactroides. Journal of Aquatic Animal Health, 28(2):114-117. DOI: https://doi.org/10.1080/08997659.2016.1152324.
Carvalho Y.B.M., Poersch L.H., Romano L.A. (2013a). Rickettsia associated mortality of the yellow clam Mesodesma mactroides (Bivalvia: Mesodesmatidae) in southern Brazil. Malacologia, 56(1-2):301-3017. DOI: https://doi.org/10.4002/040.056.0217.
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Santos J.J.S., Bernardes J.P., Ramírez J.R.B., Ramos C.O., Gomes C.H.A.M., Romano, L.A. (2020a). Embryo and larval development of the yellow clam Mesodesma mactroides (Reeve, 1854) (Mesodesmatidae) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190053. DOI: https://dx.doi.org/10.1590/0001-3765202020190053.
Santos J.J.S., Bernardes J.P., Ramírez, J.R.B., Gomes, C.H.A.M., Romano L.A. (2020b). Effect of salinity on embryo-larval development of yellow clam Mesodesma mactroides (Reeve, 1854) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190169. DOI: https://doi.org/10.1590/0001-3765202020190169.
Santos J.J.S.; Romano, L.A.; Bernardes, J.P.; Gomes, C.H.A.M. (2018). Reprodução do molusco de areia Mesodesma mactroides (Reeve, 1854) em laboratório. Aquaculture Brasil, Laguna, ED. 14ª, pp: 32-36.
Silva G. (2019). Sobrevivência e crescimento de sementes do marisco branco Amarilladesma mactroides (Reeve, 1854) em cultivo. Dissertação de mestrado, Pós-Graduação em Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Sipe J.D., Benson, M.D., Buxbaum J.N.; Ikeda S.I., Merlini G, Saraiva M.J., Westermark P. (2016). Amyloid fibril proteins and amyloidosis: chemical identification and clinical classification International Society of Amyloidosis 2016 Nomenclature Guidelines. Amyloid, 23(4): 209-213. DOI: https://doi.org/10.1080/13506129.2016.1257986.
Steel D.M., Whitehead A.S. (1994). The major acute phase reactants: C- reactive protein, serum amyloid P component and serum amyloid A protein. Immunology today, 15(2): 81-88. DOI: https://doi.org/10.1016/0167-5699(94)90138-4.
Uhlar C.M., Whitehead A.S. (1999). Serum amyloid A, the major vertebrate acute-phase reactant. European Journal of Biochemistry, 265(2): 501-523. DOI: https://doi.org/10.1046/j.1432-1327. 1999.00657.x.
Zou L., Liu B. (2015). Identification of a Serum amyloid A gene and the association of SNPs with Vibrio-resistance and growth traits in the clam Meretrix meretrix. Fish & Shellfish Immunology, 43(2): 301-309. DOI: https://doi.org/10.1016/j.fsi.2015.01.007.
Woldemeskel M.A. (2012). Concise Review of Amyloidosis in Animals. Veterinary Medicine International. 2012: 427296. DOI: https://doi.org/10.1155/2012/427296.
Bayne C.J., Gerwick L. (2001). The acute phase response and innate immunity of fish. Developmental and Comparative Immunology, 25(8-9):725-743. DOI: https://doi.org/10.1016/S0145-305X(01)00033-7.
Bibby R., Widdicombe S., Parry H., Spicer J., Pipe R. (2008). Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis. Aquatic Biology, 2(1): 67-74. DOI: https://doi.org/10.3354/ab00037.
Carvalho Y.B.M., Santos J.J.S., Raibenberg F.R., Poersch L.H., Romano L.A. (2016). Use of polymerase chain reaction for bivalve pathogen surveillance in the yellow clam Mesodesma mactroides. Journal of Aquatic Animal Health, 28(2):114-117. DOI: https://doi.org/10.1080/08997659.2016.1152324.
Carvalho Y.B.M., Poersch L.H., Romano L.A. (2013a). Rickettsia associated mortality of the yellow clam Mesodesma mactroides (Bivalvia: Mesodesmatidae) in southern Brazil. Malacologia, 56(1-2):301-3017. DOI: https://doi.org/10.4002/040.056.0217.
Carvalho Y.B.M., Poersch L.H., Junior J.P., Romano L.A. (2013b). Histopathological survey of the yellow clam Mesodesma mactroides from southern Brazil. Bulletin of the European Association of Fish Pathologists, 33(2):53-58.
Carvalho Y.B.M., Romano L.A., Poersch L.H.S. (2015). Effect of low salinity on the yellow clam Mesodesma mactroides. Brazilian Journal of Biology, 33(1):8-12. DOI: http://dx.doi.org/10.1590/1519-6984.03213.
Coscarón S. (1959). La almeja amarilla (Mesodesma mactroides, Deshayes,1854) de la costa de la Provincia de Buenos Aires. Agro Publicaciones Técnicas, 1(3):1-66.
Gagnaire B., Froin H., Moreau K., Thomas-Guyon H., Renaut T. (2006). Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg). Fish & Shellfish Immunology, 20(4):536-547. DOI: https://doi.org/10.1016/j.fsi.2005.07.003.
Jensen L.E., Whitehead, A.S. (1998). Regulation of serum amyloid a protein expression during the acute-phase response. Biochemical Journal, 334(3):489-503. DOI: https://doi.org/10.1042/bj3340489.
Odebrecht C., Bergesch M., Rörig L.R., Abreu PC. (2010). Phytoplankton interannual variability at Cassino Beach, Southern Brazil (1992-2007), with emphasis on the surf-zone diatom Asterionellopsis glacialis. Estuaries and Coasts, 33(2):570-583. DOI: https://doi.org/10.1007/s12237-009-9176-6.
Puchtler H., Sweat F., Levine M. (1962). On the binding of Congo red by amyloid. Journal of Histochemistry & Cytochemistry, 10(3):355-364. DOI: https://doi.org/10.1177/10.3.355.
Qu F., Xiang Z., Yu Z. (2014). The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization. Fish & Shellfish Immunology, 39(2):145-151. DOI: https://doi.org/10.1016/j.fsi.2014.05.013.
Rosani U., Domeneghetti S., Gerdol M., Franzoi M., Pallavicini A., Venier P. (2016). Serum amyloid A in marine bivalves: An acute phase and innate immunity protein. Developmental & Comparative Immunology, 59(2016):136-144. DOI: https://doi.org/10.1016/j.dci.2016.01.019.
Santiago-Cardona P.G., Berríos C.A., Ramírez F., García-Arrarás J.E. (2003). Lipopolysaccharides induce intestinal serum amyloid A expression in the sea cucumber Holothuria glaberrima. Developmental and Comparative Immunology, 27(2):105-110. DOI: https://doi.org/10.1016/S0145-305X(02)00068-X.
Santos J.J.S., Carvalho Y.B.M., Lopes D.L.D.A., Romano L.A. (2016). Immunological profile of the yellow clam Mesodesma mactroides (Mesodesmatidae) from the southern coast of Rio Grande do Sul, Brazil. Journal of Aquatic Animal Health, 28(1):11-20. DOI: https://doi.org/10.1080/08997659. 2015.1116471.
Santos J.J.S., Bernardes J.P., Ramírez J.R.B., Ramos C.O., Gomes C.H.A.M., Romano, L.A. (2020a). Embryo and larval development of the yellow clam Mesodesma mactroides (Reeve, 1854) (Mesodesmatidae) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190053. DOI: https://dx.doi.org/10.1590/0001-3765202020190053.
Santos J.J.S., Bernardes J.P., Ramírez, J.R.B., Gomes, C.H.A.M., Romano L.A. (2020b). Effect of salinity on embryo-larval development of yellow clam Mesodesma mactroides (Reeve, 1854) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190169. DOI: https://doi.org/10.1590/0001-3765202020190169.
Santos J.J.S.; Romano, L.A.; Bernardes, J.P.; Gomes, C.H.A.M. (2018). Reprodução do molusco de areia Mesodesma mactroides (Reeve, 1854) em laboratório. Aquaculture Brasil, Laguna, ED. 14ª, pp: 32-36.
Silva G. (2019). Sobrevivência e crescimento de sementes do marisco branco Amarilladesma mactroides (Reeve, 1854) em cultivo. Dissertação de mestrado, Pós-Graduação em Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Sipe J.D., Benson, M.D., Buxbaum J.N.; Ikeda S.I., Merlini G, Saraiva M.J., Westermark P. (2016). Amyloid fibril proteins and amyloidosis: chemical identification and clinical classification International Society of Amyloidosis 2016 Nomenclature Guidelines. Amyloid, 23(4): 209-213. DOI: https://doi.org/10.1080/13506129.2016.1257986.
Steel D.M., Whitehead A.S. (1994). The major acute phase reactants: C- reactive protein, serum amyloid P component and serum amyloid A protein. Immunology today, 15(2): 81-88. DOI: https://doi.org/10.1016/0167-5699(94)90138-4.
Uhlar C.M., Whitehead A.S. (1999). Serum amyloid A, the major vertebrate acute-phase reactant. European Journal of Biochemistry, 265(2): 501-523. DOI: https://doi.org/10.1046/j.1432-1327. 1999.00657.x.
Zou L., Liu B. (2015). Identification of a Serum amyloid A gene and the association of SNPs with Vibrio-resistance and growth traits in the clam Meretrix meretrix. Fish & Shellfish Immunology, 43(2): 301-309. DOI: https://doi.org/10.1016/j.fsi.2015.01.007.
Woldemeskel M.A. (2012). Concise Review of Amyloidosis in Animals. Veterinary Medicine International. 2012: 427296. DOI: https://doi.org/10.1155/2012/427296.
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2021-04-30
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