Presencia de proteína amiloide en la almeja amarilla Amarilladesma mactroides (Reeve, 1854), una especie emergente para la acuicultura
DOI:
https://doi.org/10.33936/at.v3i1.3439Keywords:
Amyloid protein, Yellow clam, Congo Red, Histology, InvertebratesAbstract
Amyloid proteins are proteic groups that comprise a superfamily of apolipoproteins, highly conserved in vertebrates, being involved in the modulation of numerous immune responses during infections, injuries or stress. In this study, the yellow clam Amarilladesma mactroides was used as an invertebrate model in the search for amyloid protein deposits in their tissues. Adult specimens of this bivalve were collected from the beach and transported to the Laboratory of Immunology and Pathology of Aquatic Organisms - LIPOA at the Federal University of Rio Grande – FURG. Their tissues were sectioned and stained either with Hematoxylin and Eosin, and examined using light microscopy, or with Congo Red and analyzed under polarized light microscopy. Congo Red stain revealed the presence of amyloid proteins in the foot and digestive glands of the yellow clam. The techniques used proved to be appropriate for this species and evidenced a complementary role of amyloid proteins in the immune response, indicating that they are useful as a marker for the innate immune system and health of invertebrates. The ability to assess the immunological condition can provide information related to the cultivation of emerging species for aquaculture.
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References
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Steel D.M., Whitehead A.S. (1994). The major acute phase reactants: C- reactive protein, serum amyloid P component and serum amyloid A protein. Immunology today, 15(2): 81-88. DOI: https://doi.org/10.1016/0167-5699(94)90138-4.
Uhlar C.M., Whitehead A.S. (1999). Serum amyloid A, the major vertebrate acute-phase reactant. European Journal of Biochemistry, 265(2): 501-523. DOI: https://doi.org/10.1046/j.1432-1327. 1999.00657.x.
Zou L., Liu B. (2015). Identification of a Serum amyloid A gene and the association of SNPs with Vibrio-resistance and growth traits in the clam Meretrix meretrix. Fish & Shellfish Immunology, 43(2): 301-309. DOI: https://doi.org/10.1016/j.fsi.2015.01.007.
Woldemeskel M.A. (2012). Concise Review of Amyloidosis in Animals. Veterinary Medicine International. 2012: 427296. DOI: https://doi.org/10.1155/2012/427296.
Bayne C.J., Gerwick L. (2001). The acute phase response and innate immunity of fish. Developmental and Comparative Immunology, 25(8-9):725-743. DOI: https://doi.org/10.1016/S0145-305X(01)00033-7.
Bibby R., Widdicombe S., Parry H., Spicer J., Pipe R. (2008). Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis. Aquatic Biology, 2(1): 67-74. DOI: https://doi.org/10.3354/ab00037.
Carvalho Y.B.M., Santos J.J.S., Raibenberg F.R., Poersch L.H., Romano L.A. (2016). Use of polymerase chain reaction for bivalve pathogen surveillance in the yellow clam Mesodesma mactroides. Journal of Aquatic Animal Health, 28(2):114-117. DOI: https://doi.org/10.1080/08997659.2016.1152324.
Carvalho Y.B.M., Poersch L.H., Romano L.A. (2013a). Rickettsia associated mortality of the yellow clam Mesodesma mactroides (Bivalvia: Mesodesmatidae) in southern Brazil. Malacologia, 56(1-2):301-3017. DOI: https://doi.org/10.4002/040.056.0217.
Carvalho Y.B.M., Poersch L.H., Junior J.P., Romano L.A. (2013b). Histopathological survey of the yellow clam Mesodesma mactroides from southern Brazil. Bulletin of the European Association of Fish Pathologists, 33(2):53-58.
Carvalho Y.B.M., Romano L.A., Poersch L.H.S. (2015). Effect of low salinity on the yellow clam Mesodesma mactroides. Brazilian Journal of Biology, 33(1):8-12. DOI: http://dx.doi.org/10.1590/1519-6984.03213.
Coscarón S. (1959). La almeja amarilla (Mesodesma mactroides, Deshayes,1854) de la costa de la Provincia de Buenos Aires. Agro Publicaciones Técnicas, 1(3):1-66.
Gagnaire B., Froin H., Moreau K., Thomas-Guyon H., Renaut T. (2006). Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg). Fish & Shellfish Immunology, 20(4):536-547. DOI: https://doi.org/10.1016/j.fsi.2005.07.003.
Jensen L.E., Whitehead, A.S. (1998). Regulation of serum amyloid a protein expression during the acute-phase response. Biochemical Journal, 334(3):489-503. DOI: https://doi.org/10.1042/bj3340489.
Odebrecht C., Bergesch M., Rörig L.R., Abreu PC. (2010). Phytoplankton interannual variability at Cassino Beach, Southern Brazil (1992-2007), with emphasis on the surf-zone diatom Asterionellopsis glacialis. Estuaries and Coasts, 33(2):570-583. DOI: https://doi.org/10.1007/s12237-009-9176-6.
Puchtler H., Sweat F., Levine M. (1962). On the binding of Congo red by amyloid. Journal of Histochemistry & Cytochemistry, 10(3):355-364. DOI: https://doi.org/10.1177/10.3.355.
Qu F., Xiang Z., Yu Z. (2014). The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization. Fish & Shellfish Immunology, 39(2):145-151. DOI: https://doi.org/10.1016/j.fsi.2014.05.013.
Rosani U., Domeneghetti S., Gerdol M., Franzoi M., Pallavicini A., Venier P. (2016). Serum amyloid A in marine bivalves: An acute phase and innate immunity protein. Developmental & Comparative Immunology, 59(2016):136-144. DOI: https://doi.org/10.1016/j.dci.2016.01.019.
Santiago-Cardona P.G., Berríos C.A., Ramírez F., García-Arrarás J.E. (2003). Lipopolysaccharides induce intestinal serum amyloid A expression in the sea cucumber Holothuria glaberrima. Developmental and Comparative Immunology, 27(2):105-110. DOI: https://doi.org/10.1016/S0145-305X(02)00068-X.
Santos J.J.S., Carvalho Y.B.M., Lopes D.L.D.A., Romano L.A. (2016). Immunological profile of the yellow clam Mesodesma mactroides (Mesodesmatidae) from the southern coast of Rio Grande do Sul, Brazil. Journal of Aquatic Animal Health, 28(1):11-20. DOI: https://doi.org/10.1080/08997659. 2015.1116471.
Santos J.J.S., Bernardes J.P., Ramírez J.R.B., Ramos C.O., Gomes C.H.A.M., Romano, L.A. (2020a). Embryo and larval development of the yellow clam Mesodesma mactroides (Reeve, 1854) (Mesodesmatidae) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190053. DOI: https://dx.doi.org/10.1590/0001-3765202020190053.
Santos J.J.S., Bernardes J.P., Ramírez, J.R.B., Gomes, C.H.A.M., Romano L.A. (2020b). Effect of salinity on embryo-larval development of yellow clam Mesodesma mactroides (Reeve, 1854) in laboratory. Anais da Academia Brasileira de Ciências, 92(Suppl.1): e20190169. DOI: https://doi.org/10.1590/0001-3765202020190169.
Santos J.J.S.; Romano, L.A.; Bernardes, J.P.; Gomes, C.H.A.M. (2018). Reprodução do molusco de areia Mesodesma mactroides (Reeve, 1854) em laboratório. Aquaculture Brasil, Laguna, ED. 14ª, pp: 32-36.
Silva G. (2019). Sobrevivência e crescimento de sementes do marisco branco Amarilladesma mactroides (Reeve, 1854) em cultivo. Dissertação de mestrado, Pós-Graduação em Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Sipe J.D., Benson, M.D., Buxbaum J.N.; Ikeda S.I., Merlini G, Saraiva M.J., Westermark P. (2016). Amyloid fibril proteins and amyloidosis: chemical identification and clinical classification International Society of Amyloidosis 2016 Nomenclature Guidelines. Amyloid, 23(4): 209-213. DOI: https://doi.org/10.1080/13506129.2016.1257986.
Steel D.M., Whitehead A.S. (1994). The major acute phase reactants: C- reactive protein, serum amyloid P component and serum amyloid A protein. Immunology today, 15(2): 81-88. DOI: https://doi.org/10.1016/0167-5699(94)90138-4.
Uhlar C.M., Whitehead A.S. (1999). Serum amyloid A, the major vertebrate acute-phase reactant. European Journal of Biochemistry, 265(2): 501-523. DOI: https://doi.org/10.1046/j.1432-1327. 1999.00657.x.
Zou L., Liu B. (2015). Identification of a Serum amyloid A gene and the association of SNPs with Vibrio-resistance and growth traits in the clam Meretrix meretrix. Fish & Shellfish Immunology, 43(2): 301-309. DOI: https://doi.org/10.1016/j.fsi.2015.01.007.
Woldemeskel M.A. (2012). Concise Review of Amyloidosis in Animals. Veterinary Medicine International. 2012: 427296. DOI: https://doi.org/10.1155/2012/427296.
Published
2021-04-30
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Nota Científica
