Growth and alkaline digestive proteases activity of Parachromis dovii larvae fed live prey and formulated diets with different protein sources

Autores/as

Juan Bautista Ulloa Rojas Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica https://orcid.org/0000-0003-4464-1136
Mauricio Herrera Campos Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica http://orcid.org/0000-0003-3327-1950
Silvia Valverde Chavarria Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica https://orcid.org/0000-0003-2823-459X

DOI:

https://doi.org/10.33936/at.v4i2.4610

Palabras clave:

Fish larvae, Protein sources, Enzymatic activity, Feeding

Resumen

ABSTRACT The Parachromis dovii is a carnivorous fish from Central America, which easily accept formulated feeds since exogenous feeding starts; however, its larval growth is lower than that obtained using live food. For this reason, the effect of different dietary protein source combinations on growth and alkaline digestive proteases activity were tested in P. dovii larvae. The test protein mixtures were prepared using fish, tankage and poultry by-product meals in different proportions (four diets) and compared to a commercial product (Artemia cysts Vitellus) and Artemia nauplii as a control. At the end of the trial, larvae fed Artemia nauplii showed the best growth (specific growth rate (SGR): 17.6% BW/d), contrary the Vitellus diet presented the lowest SGR (12.0% body weight per day (BW/d)) (LSD, P<0.05). From the protein sources, D3 (with the highest content poultry by-products meal) showed the worst SGR (12.6% BW/d) while the other treatments gave similar values (D1: 13.5%, D2: 13.2% and D4: 13.7% BW/d). Survival was not affected by dietary treatment (over 93%). The lowest alkaline digestive protease activity was found at six days after hatching (DAH), and it was similar between treatments (P>0.05). The protease activity increased up to 27 DAH, being higher with larvae fed Artemia than other treatments (P<0.05). There was no difference in protease activity between the protein mixture diets, and there was no difference between the formulated diets and Vitellus, except at 20 DAH (P<0.05).

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