Crecimiento y actividad de proteasas alcalinas digestivas de larvas de Parachromis dovii alimentadas con presas vivas y dietas formuladas con diferentes fuentes proteicas

Authors

Juan Bautista Ulloa Rojas Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica https://orcid.org/0000-0003-4464-1136
Mauricio Herrera Campos Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica http://orcid.org/0000-0003-3327-1950
Silvia Valverde Chavarria Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, 86 - 3000 Heredia, Costa Rica https://orcid.org/0000-0003-2823-459X

DOI:

https://doi.org/10.33936/at.v4i2.4610

Keywords:

Fish larvae, Protein sources, Enzymatic activity, Feeding

Abstract

RESUMO O Parachromis dovii é um peixe carnívoro da América Central, o qual aceita facilmente rações formuladas desde o início da alimentação exógena; no entanto, seu crescimento larval é inferior ao obtido com alimentos vivos. Por esta razão, o efeito de diferentes combinações de fontes de proteína na dieta sobre o crescimento e a atividade de proteases digestivas alcalinas foi testado em larvas. As misturas proteicas de teste foram preparadas utilizando farinhas de peixe e subprodutos de aves em diferentes proporções (quatro dietas) e comparadas com um produto comercial (Artemia cysts Vitellus) e náuplios de Artemia, como controle. No final do ensaio, as larvas alimentadas com náuplios de Artemia apresentaram o melhor crescimento (taxa de crescimento específico (TCE): 17,6% PC/d). Por outro lado, a dieta Vitellus apresentou o menor TCE (12,0% peso corporal por dia (PC/d)) (LSD, P<0,05). Das fontes proteicas, o D3 (com o maior teor de farinha de subprodutos de aves) apresentou o pior TCE (12,6% PC/d), enquanto os demais tratamentos apresentaram valores similares (D1: 13,5%, D2: 13,2% e D4: 13,7% BW/d). A sobrevivência não foi afetada pelo tratamento (mais de 93%). A menor atividade de protease digestiva alcalina foi encontrada aos seis dias após a eclosão (DAH), e foi similar entre os tratamentos (P>0,05). A atividade da protease aumentou até 27 DAH, sendo maior nas larvas alimentadas com Artemia do que nos outros tratamentos (P<0,05). Não houve diferença na atividade da protease entre as dietas com mistura proteica e não houve diferença entre as dietas formuladas e a Vitellus, exceto aos 20 DAH (P<0,05).

Downloads

Download data is not yet available.

References

Aguilera, C., Mendoza, R., Iracheta, I., Márquez, G. (2012). Digestive enzymatic activity on tropical gar (Atractosteus tropicus) larvae fed different diets. Fish Physiology and Biochemistry, 38:6779-691.

Alarcón, F., García-Carreño, F., Navarrete del Toro, M. (2001). Effect of plant protease inhibitors on digestive proteases in two fish species, Lutjanus argentiventris and L. novemfasciatus. Fish Physiology and Biochemistry, 24:179-189.

Alla, Y., Célestin, B., Célestin, A., Tidiani, K. (2011). Effect of three diets on growth and survival rates of African catfish Heterobranchus bidorsalis larvae. The Israeli Journal of Aquaculture-Bamidgeh, 63:1-8.

AOAC (Association of Official Analytical Chemists). 1990. Official Methods of Analysis of the Association of Official Analytical Chemists. Food Composition; Additives; Natural Contaminants. Ed. by K. Helrich. 15th edition. Virginia, USA. 1298 p.

Bussing, W. (2002). Peces de las Aguas Continentales de Costa Rica. Editorial de la Universidad de Costa Rica. San José, Costa Rica.

Drossou, A., Ueberschär, B., Rosenthal, H., Herzig, K. (2006). Ontogenic development of the proteolytic digestion activities in larvae of Oreochromis niloticus fed with different diets. Aquaculture, 256:479-488.

García-Ortega, A., Verreth, J., Segner, H. (2000). Post-prandial protease activity in the digestive tract of African catfish Clarias gariepinus larvae fed decapsulated cysts of Artemia. Fish Physiology and Biochemistry, 22:237-244.

Giguere, M.A. (2011). The economics of partial Artemia replacement using two commercially available feed in diets of Litopenaeus vannamei from Z3/M1 – PL10. Master's tesis, University of Miami, Florida, USA.

Günther, J. (1996). Crecimiento del guapote lagunero (Cichlasoma dovii) en régimen de cultivo intensivo en estanques y su dependencia de la densidad. UNICIENCIA, 13:13-19.

Hassantabar, F., Fereidoumi, A., Ouraji, H., Babaei, S., Jafarpour, A. (2015). Comparison of growth and digestive enzymes activity of kutum (Rutilus kutum) during ontogeny fed with live prey and artificial feed. Aquaculture International, 23:597-612.

Kamarudin, M., Otoi, S., Saad, R. (2011). Changes in growth, survival and digestive enzyme activities of Asian redtail catfish, Mystus nemurus, larvae fed on different diets. African Journal of Biotechnology, 10:4484-4493.

Kolkovski, S. (2001). Digestive enzymes in fish larvae and juveniles-implications and applications to

formulated diets. Aquaculture, 200:181-201.

Kolkovski, S., Koven, W., Tandler, A. (1997). The mode of action of Artemia in enhancing utilization of

microdiet by gilthead seabream Sparus aurata larvae. Aquaculture, 155:193-205.

Lazo, J. (2000). Conocimiento actual y nuevas perspectivas en el desarrollo de dietas para larvas de peces

marinos. Avances en Nutrición Acuícola V. Mérida, Yucatán, México (noviembre 2000).

Lazo, J., Darias, M., Gisbert, E. (2011). Ontogeny of the digestive tract. In: Holt G (ed). Larval fish nutrition.

John Wiley and Sons. London, United Kingdom. pp: 1-46.

Lo, M., Weng, C. (2006). Developmental regulation of gastric pepsin and pancreatic serine protease in larvae

of the euryhaline Oreochromis mossambicus. Aquaculture, 261:1403-1412.

López-Ramírez, G., Cuenca-Soria, C. Álvarez-González, C., Tovar-Ramírez, D., Ortiz-Galindo, J. L., Perales-García, N., Márquez-Couturier, G., Arias-Rodríguez, L., Indy, J.R., Contreras-Sánchez, W. M., Gisbert, E., Moyano, F. J. (2011). Development of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus. Fish Physiology and Biochemistry, 37:197-208.

Martínez-Montaño, E., Lazo, J. (2012). In vitro protein digestibility of dietary ingredients throughout ontogeny of California halibut, Paralichtys californicus, larvae. Journal of the World Aquaculture Society, 43:51–62.

Mente, E., Solovyev, M., Vlahos, N., Rotllant, G. and Gisbert, E. (2017). Digestive enzyme activity during ontogeny and after feeding diets with different protein sources in zebra cichlid, Archocentrus nigrofasciatus. Journal of the World Aquaculture Society, 48:831-848.

Murashita, K., Fukada, H., Takahashi, N., Hosomi, N., Matsunari, H., Furuita, H., Oku, H., Yamamoto, T. (2015). Effect of feed ingredients on digestive enzyme secretion in fish. Bulletin of Fisheries Research Agency, 40:69-74.

Noori, F., Van Stappen, G., Sorgeloos, P. (2012). Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipenser persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. Aquaculture Research, 43:198-207.

Olurin, K., Oluwo, A. (2010). Growth and survival of African catfish (Clarias gariepinus) larvae fed decapsulated Artemia, live Daphnia, or commercial starter diet. The Israeli Journal of Aquaculture- Bamidgeh, 62:50-55.

Quirós, J., Valverde, S., Ulloa, J. (2014). The proteolytic digestive activity and growth during ontogeny of Parachromis dovii larvae (Pisces: Cichlidae) using two feeding protocols. Fish Physiology and Biochemistry, 40:1253–1261.

Szkudlarek, M., Zakęś, Z. (2007). Effect of stocking density on survival and growth performance of pikeperch, Sander lucioperca (L.), larvae under controlled conditions. Aquaculture International, 15:67– 81.

Tengjaroenkul, B., Smith, B., Smith, S., Chatreewongsin, U. (2002). Ontogenic development of the intestinal enzymes of cultured Nile tilapia, Oreochromis niloticus L. Aquaculture, 211:241-251.

Toledo-Solís, F., Uscanga-Martínez, A., Guerrero-Zárate, R., Márquez-Couturier, G., Martínez-García, R., Camarillo-Coop, S., Perales-García, N., Rodríguez-Valencia, W., Gómez-Gómez, M., Álvarez-González,

C. (2015). Change on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma

trimaculatum. Fish Physiology and Biochemistry, 41:267-279.

Uscanga-Martínez, A., Moyano-López, F., Álvarez-González, C., Perales-García, N. (2011). Aplicaciones a la mejora de la utilización nutritiva del alimento en cíclidos cultivados en México. Avances en Nutrición Acuícola XI. Monterrey, México (noviembre 2011).

Valverde-Chavarría, S., Álvarez-González, C. A., Brais-Medina, M., Calvo-Elizondo, E., Ulloa-Rojas, J. B. (2016). In vitro digestibility and proteases inhibitory effect of several feedstuffs for Parachromis dovii juveniles and P. dovii hybrid larvae. Fish Physiology and Biochemistry, 42:1767-1776.

Valverde-Chavarría, S., Álvarez-González, C., Ulloa-Rojas, J., Frías-Quintana, C.A., Guerrero-Zárate, R., Quirós-Orlich, J.R., Brais-Medina, M., Calvo-Elizondo, E., Alvarado-Guzmán, L. (2013). Ontogenia del sistema digestivo del guapote lagunero Parachromis dovii durante el periodo larval y selección de ingredientes para su alimentación. Avances en Nutrición Acuícola XII. Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México (noviembre 2013).

Walter, H. (1984). Proteinases: methods with hemoglobin, casein and azocoll as substrates. In: Bergmeyern, H. (Ed). Methods of Enzymatic Analysis. Vol. V. Verlag Chemic Weinheim, Germany. pp: 270-277.