Efeito da dieta com compostos bioativos de origem vegetal nas respostas fisiológicas e teciduais da carpa comum (Cyprinus carpio)
Aquicultura e pesca
DOI:
https://doi.org/10.33936/latecnica.v14i2.6194Palavras-chave:
antioxidantes; fenóis; casca de romã; carpa comum.Resumo
O objetivo deste estudo foi investigar algumas respostas fisiológicas à influência do epicarpo (casca) de romã crua (Punica granatum) (RPP) e de seu extrato alcoólico (PPE) em parâmetros hematológicos, respostas imunológicas e antioxidantes, além da análise histológica em alevinos de carpa comum (Cyprinus carpio) (13,5 ± 0,1 g). Os tratamentos incluíram duas concentrações de RPP e PPE (controle, 0,5 e 1 mg·kg⁻¹ de dieta) incorporadas à dieta comercial (~35% de proteína) durante 70 dias. Ao final do experimento, todos os peixes foram anestesiados para medições morfométricas e coleta de amostras sanguíneas. Foram observados aumentos significativos nos níveis de hemoglobina (HGB), hematócrito (Hct) e contagem de glóbulos vermelhos (RBCs) nos peixes alimentados com RPP e PPE na dieta (P<0,05). A atividade da lisozima melhorou significativamente em todas as dietas que continham PPE, em comparação com o grupo controle (P<0,05). Também foi observada uma melhora na atividade da catalase e da glutationa peroxidase (GPx) (P<0,05). A análise histológica revelou um fígado com estrutura normal e presença de vacúolos lipídicos. Os achados deste estudo indicam uma melhoria geral nos parâmetros sanguíneos, na resposta imune, no estado antioxidante e na manutenção da eficiência hepática com a inclusão de PPE na dieta da carpa comum.
Downloads
Referências
Abdel-Moneim, A. E. (2012). Evaluating the potential role of pomegranate peel in aluminum-induced oxidative stress and histopathological alterations in brain of female rats. Biological Trace Element Research, 150(1), 328-336.
Akhtar, S., Ismail, T., Fraternale, D. and Sestili, P. (2015). Pomegranate peel and peel extracts: Chemistry and food features. Food Chemistry, 174, 417-425.
Altieri, F., Cairone, F., Giamogante, F., Carradori, S., Locatelli, M., Chichiarelli, S. and Cesa, S. (2019). Influence of ellagitannins extracted by pomegranate fruit on disulfide isomerase PDIA3 activity. Nutrients, 11(1), 186. https://doi.org/10.3390/nu11010186
Ambigaipalan, P., De Camargo, A. C. and Shahidi F. (2016). Phenolic compounds of pomegranate byproducts (outer skin, mesocarp, divider membrane) and their antioxidant activities. Journal of Agricultural and Food Chemistry, 64(34), 6584-6604. https://doi.org/10.1021/acs.jafc.6b0 2950
Ardekani, M. R. S., Hajimahmoodi, M., Oveisi, M. R., Sadeghi, N., Jannat, B., Ranjbar, A. M., Gholam, N. and Moridi, T. (2011). Comparative antioxidant activity and total flavonoid content of persian pomegranate (Punica granatum L.) cultivars. Iranian Journal of Pharmaceutical Research, 10(3), 519. https://doi.org/ 10.22037/ijpr.2011.948
Ayadi, I., Monteiro, S. M., Regaya, I., Coimbra, A., Fernandes, F., Oliveira, M. M., Peixoto, F. and Mnif, W. (2015). Biochemical and histological changes in the liver and gills of Nile tilapia Oreochromis niloticus exposed to Red 195 dye. RSC Advances, 5(106), 87168-87178. https://doi.org/10.1039/C5RA13127H
Badawi, M. E. and Gomaa, A. M. (2016). Influence of diets supplemented with pomegranate peel extract on performance in Oreochromus niloticus. Japanese Journal of Veterinary Research, 64 (Supplement 2), S87-S94. http://hdl.handle.net/2115/62021
Badrey, A. A., Osman, A., Farrag, S. M., Toutou, M. M. M. and Moustafa, M. (2019). Influences of diets supplemented with pomegranate peel on haematology, blood biochemistry and immune status in monosex Nile tilapia, Oreochromis niloticus. Egyptian Journal of Aquatic Biology and Fisheries, 23(2), 133-144. https://dx.doi.org/ 10.21608/ejabf.2019. 33559
Banan Khojasteh, S. M. (2012). The morphology of the post-gastric alimentary canal in teleost fishes: a brief review. International Journal of Aquatic Science, 3(2), 71-88.
Bonvini, E. (2017). Development of innovative feed products and feed concepts for marine species in aquaculture. Ph.D. Thesis. University of Bologna. 100 p.
Bancroft, J. D. and Layton, C. (2013). The hematoxylin and eosin. pp. 172-214. In: Suvarna, S. K., Layton, C. and Bancroft, J. D. (Eds.). Theory and practice of histological techniques. 7th Ed., Churchill Livingstone of El Sevier, Philadelphia, Ch. 10 and 11.
Banihani, S., Swedan, S. and Alguraan, Z. (2013). Pomegranate and type 2 diabetes. Nutrition Research, 33(5), 341-348.https://doi.org/10.1016/j.nutres.2013.03.003
Bassiri-Jahromi, S. (2018). Punica granatum (Pomegranate) activity in health promotion and cancer prevention. Oncology Reviews, 12(1), 345,351. https://doi.org/ 10.4081%2Foncol. 2018.345
Caballero, M. J., López-Calero, G., Socorro, J., Roo, F. J., Izquierdo, M. S. and Fernández, A. J. (1999). Combined effect of lipid level and fish meal quality on liver histology of gilthead seabream (Sparus aurata). Aquaculture, 179(1-4), 277-290. https://doi.org/10.1016/S0044-8486 (99)00165-9
Christaki, E., Giannenas, I., Bonos, E. and Florou-Paneri, P. (2020). Innovative uses of aromatic plants as natural supplements in nutrition. pp. 19-34. In: Florou-Paneri, P., Christaki, E. and Giannenas, I. (Eds.). Feed additives. Cambridge, Massachusetts. Academic Press.
Chun, O. K., Kim, D. O., Moon, H. Y., Kang, H. G. and Lee, C. Y. (2003). Contribution of individual polyphenolics to total antioxidant capacity of plums. Journal of Agricultural and Food Chemistry, 51(25), 7240-7245. https://doi.org/ 10.1021/jf0343579
Dahham, S. S., Ali, M. N., Tabassum, H. and Khan, M. (2010). Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). American-Eurasian Journal of Agricultural and Environmental Sciences, 9(3), 273-281.
Danesi, F. and Ferguson, L. R. (2017). Could pomegranate juice help in the control of inflammatory diseases?. Nutrients, 9(9), 958. https://doi.org/10.3390/nu9090958
Everly, G. S. and Lating, J. M. (2013). The anatomy and physiology of the human stress response. pp. 17-51. In: Everly, G. S. and Lating J. M. (Eds.). A clinical guide to the treatment of the human stress response. Springer, New York, NY.
Gülçın, İ., Oktay, M., Kıreçcı, E. and Küfrevıoǧlu, Ö. İ. (2003). Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem., 83(3), 371-382.
Hamed, H. S. and Abdel-Tawwab, M. (2021). Dietary pomegranate (Punica granatum) peel mitigated the adverse effects of silver nanoparticles on the performance, haemato-biochemical, antioxidant, and immune responses of Nile tilapia fingerlings. Aquaculture, 540, 736742. https://doi.org/10.1016/j.aquaculture.2021.736742
Harikrishnan, R., Kim, J. S., Kim, M. C., Balasundaram, C. and Heo, M. S. (2012). Pomegranate enriched diet enhances the hematology, innate immune response, and disease resistance in olive flounder against Philasterides dicentrarchi. Veterinary Parasitology, 187(1-2), 147-156. https://doi.org/10.1016/j.vetpar.2011.12.006
Hou, C., Zhang, W., Li, J., Du, L., Lv, O., Zhao, S. and Li, J. (2019). Beneficial effects of pomegranate on lipid metabolism in metabolic disorders. Molecular Nutrition and Food Research, 63(16), 1800773. https://doi.org/ 10.1002/mnfr.201800773
Hussein, E. E., Fatan, N., El-Betagy, A. E. D. and Naeem, M. A. (2022). Enhancement of the nutritional value, antioxidant properties and growth performance in mono-sex Nile tilapia, Oreochromis niloticus fries by pomegranate peel supplementation. ResearchSquare. https://doi.org/ 10.21203/rs.3.rs-1717922/v1
Kaderides, K., Goula, A. M. and Adamopoulos, K. G. (2015). A process for turning pomegranate peels into a valuable food ingredient using ultrasound-assisted extraction and encapsulation. Innovative Food Science and Emerging Technologies, 31, 204-215. https://doi.org/10.1016/j.ifset.2015.08.006
Lebelo, S. L., Saunders, D. K. and Crawford, T. G. (2001). Observations on blood viscosity in striped bass, Morone saxatilis (Walbaum) associated with fish hatchery conditions. Kansas Academy of Science, 104, 183194. https://doi.org/10.1660/0022-8443(2001)104[0183: OOBVIS]2.0.CO;2
Li, Y., Guo, C., Yang, J., Wei, J., Xu, J. and Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96(2), 254-260. https://doi.org/ 10.1016/j.foodchem.2005.02.033
Lin, J. Y. and Tang, C. Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1), 140-147.
Mashkor, I. M. A. A. and Muhson, A. A. (2014). Total phenol, total flavonoids and antioxidant activity of pomegranate peel. International Journal of ChemTech Research, 6(11), 4656-4661.
Monir, W., Abdel-Rahman, M. A., Hassan, S. E. D. and Awad, S. M. (2020). Pomegranate peel and moringa-based diets enhanced biochemical and immune parameters of Nile tilapia against bacterial infection by Aeromonas hydrophila. Microbial Pathogenesis, 145, 104202. https://doi.org/10.1016/j.micpath.2020.104202
Moskaug, J. Ø., Carlsen, H., Myhrstad, M. C. and Blomhoff, R. (2005). Polyphenols and glutathione synthesis regulation. The American Journal of Clinical Nutrition, 81(1), 277S-283S.
Nuamsetti, T., Dechayuenyong, P. and Tantipaibulvut, S. (2012). Antibacterial activity of pomegranate fruit peels and arils. Science Asia, 38(3), 319-322.
Orak, H. H., Yagar, H. and Isbilir, S. S. (2012). Comparison of antioxidant activities of juice, peel, and seed of pomegranate (Punica granatum L.) and inter-relationships with total phenolic, tannin, anthocyanin, and flavonoid contents. Food Science and Biotechnology, 21(2), 373-387. https://doi.org/10.1007/s10068-012-0049-6
Pickering, A.D. (1993). Growth and stress in fish production. In Genetics in aquaculture. Aquaculture, 111, 51-63. https://doi.org/10.1016/B978-0-444-81527-9.50010-5
Reverter, M., Tapissier-Bontemps, N., Sarter, S., Sasal, P. and Caruso, D. (2021). Moving towards more sustainable aquaculture practices: A meta-analysis on the potential of plant-enriched diets to improve fish growth, immunity and disease resistance. Review in Aquaculture, 13(1), 537-555. https://doi.org/10.1111/raq.12485
Sönmez, A. Y., Bilen, S., Özdemir, K. Y., Alagöz, K. and Özçelik, H. (2022). Effect of aqueous methanolic extract of pomegranate peel (Punica granatum) and veratrum (Veratrum album) on oxidative status, immunity and digestive enzyme activity in rainbow trout (Oncorhynchus mykiss). Journal of Agricultural Sciences, 28(2), 159-170. https://doi.org/ 10.15832/ankutbd.870923
Suphoronski, S. A., Chideroli, R., Facimoto, C. T., Mainardi, R. M., Souza, F. P. and Lopera-Barrero, N. M. (2019). Effects of a phytogenic, alone and associated with Potassium diformate, on tilapia growth, immunity, gut microbiome and resistance against Francisellosis. Scientific Reports, 9(1), 6045. https://doi.org/10.1038/s41598-019-42480-8
Suljević, D., Alijagić, A., Mitrašinović-Brulić, M., Fočak, M. and Islamagić, E. (2016). Comparative physiological assessment of common carp (Cyprinus carpio) and crucian carp (Carassius carassius) based on electrolyte and hematological analysis. Macedonian Journal of Animal Science, 6(2), 95-100. http://dx.doi.org/10.54865/mjas1662095s
Tortora, K., Femia, A. P., Romagnoli, A., Sineo, I., Khatib, M., Mulinacci, N., Giovannelli, L. and Caderni, G. (2018). Pomegranate by‐products in colorectal cancer chemoprevention: effects in apc‐mutated pirc rats and mechanistic studies in vitro and ex vivo. Molecular Nutrition & Food Research, 62(2), 1700401. https://doi.org/10.1002/mnfr.201700401
Türkyılmaz, M., Tağı, Ş., Dereli, U. and Özkan, M. (2013). Effects of various pressing programs and yields on the antioxidant activity, antimicrobial activity, phenolic content and colour of pomegranate juices. Food Chemistry, 138(2-3): 1810-1818. https://doi.org/10.1016/j. foodchem.2012.11.100
Zhishen, J., Mengcheng, T. and Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2024 Raad, Fatima A. M. Sultan , Riyadh A. Al-Tameemi
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
