Effect of two commercial probiotics on weight gain, hematological parameters and intestinal histology in the Pacific fat sleeper Dormitator latifrons
DOI:
https://doi.org/10.33936/at.v2i1.2408Keywords:
Instestine, blood, tratamients, food, controlAbstract
Probiotics in aquaculture are well known for their beneficial effects on the digestive tract and immune system of hosts; however, very little is known about their application in Dormitator latifrons, a species with great aquaculture potential. In that sense, the objective of this study was to evaluate the effect of two commercial probiotics on weight gain, blood parameters and histological changes in the intestinal tissue of the Dormitator latifrons. We implemented a randomized experimental design consisting of a control and two treatments, with three replicas each, and a duration of 22 days. All organisms (n= 45) with an initial average weight of 20.8 ± 7.5 g, were fed commercial tilapia concentrate at 35% protein at a rate of 3% biomass. To treatments 1 (T1) and 2 (T2) probiotics were added in concentrations of 2 X 109 CFU/g and 6 X 109 CFU/g, respectively, administered in the feed. No probiotics were administered to the control group. Water quality parameters (temperature, salinity, pH and oxygen) were recorded daily and the dead organisms in each experimental unit were counted. At the end of the experiment, each organism was determined blood parameters (hematocrit (%), hemoglobin (g/dL) and differential count), and five of each treatment were sacrificed and intestinal tissue samples were taken, which were fixed in 10% neutral formalin for histological analysis. In spite of not observing significant differences between the treatments (p> 0,05), a clear tendency is observed that the T1 presents better results is in terms of growth, weight gain and survival (60%) and in the blood and histological parameters regarding the length of the villi. Although preliminary, the results are encouraging in terms of the advantages of using commercial probiotics in chames to improve their culture.Downloads
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References
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Reda R., Selim K. (2015). Evaluation of Bacillus amyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus. Aquaculture International, 23:203–217. https://doi.org/10.1007/s10499-014-9809-z
Zar J. (2010). Biostatistical Analysis (5th ed. pp:994) Englewood Cliff, NJ: Prentice Hall.
Caspary W. (1992). Physiology and pathophysiology of intestinal absorption. The American Journal of Clinical Nutrition, 55:299S-308S.
Centeno A., Reyes J. (2009). Enfermedades del chame (Dormitator latifrons) en cultivo. Tesis de pregrado, Escuela de Acuicultura y Pesquería, Universidad Técnica de Manabí, Bahía de Caráquez, Ecuador. pp:150.
Csirke B. (1980). Introducción a la dinámica de poblaciones de peces. (N° 192). Manual Técnico de la FAO. Roma, Italia. pp: 82.
Delgadillo A., Martínez C., Berruecos J., Ulloa R., López R., Vásquez C. (2012). Caracterización de la curva de crecimiento en dos especies de pez blanco Chirostoma estor, C. promelas y sus híbridos. Veterinaria México, 43:113-121.
Elsabagh, M., Mohamed R., Moustafa, E., Hamza A., Farrag, F., Decamp O., Dawood M., Eltholth, M. (2018). Assessing the impact of Bacillus strains mixture probiotic on water quality, growth performance, blood profile and intestinal morphology of Nile tilapia, Oreochromis niloticus. Aquaculture nutrition, 24:1613-1622.
FAO (2011). Peces nativos de agua dulce de América del Sur de interés para la acuicultura: Una síntesis del estado de desarrollo tecnológico de su cultivo. ISBN 978-92-5-306658-2, pp:63-70. http://www.fao.org/3/i1773s/i1773s00.htm
Freire L. (2001). Enfermedades del Chame. Revista Raíces Productivas, 44:32-33.
Froese R. (2006). Cube law, condition factor and weight–length relationships: history, meta‐analysis and recommendations. Journal of Applied Ichthyology, 22:241-253. https://doi.org/10.1111/j.1439-0426.2006.00805.x
Gisbert E., Castillo M., Skalli A., Andree K., Badiola I. (2013) Bacillus cereus var. toyoi promotes growth, affects the histological organization and microbiota of the intestinal mucosa in rainbow trout fingerlings. Journal of Animal Science, 91:2766–2774.
Harper G., Saoud I., Emery M., Mustafa S., Rawling M., Eynon B., Davies S., Merrifield D., Monfort M. (2011) An ex vivo approach to studying the interactions of probiotic Pediococcus acidilactici and Vibrio (Listonella) anguillarum in the anterior intestine of rainbow trout Oncorhynchus mykiss. Journal of Aquaculture Research and Development S1(4):2-6. https://doi.org/doi:10.4172/2155-9546.S1-004
Humason G. (1979). Animal tissue techniques, 4th Edition. San Francisco, Estados Unidos. pp: 661.
Lara F., Escobar L., Olvera M. (2002). Avances en la utilización de probióticos como promotores de crecimiento en tilapia nilótica (Oreochromis niloticus). (en línea). Mérida Yucatán, MX. Consultado 09 mayo 2019. Disponible en: http://www.uanl.mx/utilerias/nutricion_acuicola/VI/archivos/A22.pdf.
Malachowski E., Romanowsky D. (1890). On the question of the structure of malaria parasites. Vrach 11:
1171-1173. http://www.rom anow sky.ru/site files/docs/Rom anow sky/rom a nowsky1(orig).pdf (accessed January 2019) [Russian]; http://www.romanowsky.ru/sitefiles/docs/ Romanowsky/romanowsky1(en).pdf
Martínez D. (2011). Efectos de la administración del probiótico Shewanella pdp 11 en juveniles de lenguado senegalés (Solea Senegalensis, Kaup 1858) y desarrollo de un método para su microencapsulación. Tesis de pregrado, Universidad de Almería. pp:98.
Martínez T. (2012). Manual de prácticas de laboratorio “Biometría Hemática”. México, México. pp:57. Mello H., Moraes J., Niza I., Moraes F., Ozório R., Shimada M., Filho E., Jair R., Claudiano G. (2013).
Efeitos benéficos de probióticos no intestino de juvenis de Tilápia-do-Nilo. Pesquisa Veterinária Brasileira, 33:724–730. https://doi.org/10.1590/S0100-736X2013000600006
Merrifield D., Bradley G., Baker R., Dimitroglou A., Davies S. (2010a) Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) I. Effects on growth performance, feed utilisation, intestinal microbiota and related health criteria. Aquaculture Nutrition., Early View. 16(5):504-510. https://doi.org/10.1111/j.1365-2095.2009.00689.x.
Merrifield D., Harper G., Dimitroglou A., Ringø E., Davies S. (2010b). Possible influence of probiotic adhesion to intestinal mucosa on the activity and morphology of rainbow trout (Oncorhynchus mykiss) enterocytes. Aquaculture Research, 41:1268-1272.
Merrifield D., Bradley G., Baker R., Davies S. (2010c). Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. Effects on growth performance, feed utilization intestinal microbiota and related health criteria postantibiotic treatment. Aquaculture Nutrition, 16:496-503.
Nayak S. (2010). Probiotics and immunity: A fish perspective. Fish & Shellfish Immunolology, 29:2–14. https://doi.org/10.1016/j.fsi.2010.02.017
Nutrivet. (2009). (Nutrición Veterinaria, GT) información técnica del Bacillus subtilis y del producto BIOTEC Guatemala, GT, Nutrivet (trifolio).
Raida M., Larsen J., Nielsen M., Buchmann K. (2003). Enhanced resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), against Yersinia ruckeri challenge following oral administration of Bacillus subtilis and B. licheniformis (BioPlus2B). Journal of Fish diseases, 26:495–498.
Ramos M., Batista S., Pires M., Silva A., Pereira L., Saavedra M., Ozório R., Rema P. (2017). Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia. Animal, 11: 1259–1269. https://doi.org/10.1017/S1751731116002792
Reda R., Selim K. (2015). Evaluation of Bacillus amyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus. Aquaculture International, 23:203–217. https://doi.org/10.1007/s10499-014-9809-z
Zar J. (2010). Biostatistical Analysis (5th ed. pp:994) Englewood Cliff, NJ: Prentice Hall.
Published
2020-05-13
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