Effect of two probiotics on survival, growth and protein concentration in hemolymph of Penaeus vannamei Boone 1931 confronted with ionic imbalance

Authors

Alexander Javier Basurto Aguirre Facultad de Posgrado, Universidad Técnica de Manabí, Bahía de Caráquez, Manabí, Ecuador.
Alexandra Elizabeth Bermúdez-Medranda Departamento de Acuicultura, Pesca y Recursos Naturales Renovables. Grupo de Investigación en Sanidad Acuícola, Inocuidad y Salud Ambiental. Facultad de Acuicultura y Ciencias del Mar. Universidad Técnica de Manabí Bahía de Caráquez, Manabí EC130104, Ecuador. https://orcid.org/0000-0002-5451-3990
Yanis Cruz Quintana Departamento de Acuicultura, Pesca y Recursos Naturales Renovables. Grupo de Investigación en Sanidad Acuícola, Inocuidad y Salud Ambiental. Facultad de Acuicultura y Ciencias del Mar. Universidad Técnica de Manabí Bahía de Caráquez, Manabí EC130104, Ecuador.
Juan Carlos Vélez-Chica Departamento de Acuicultura, Pesca y Recursos Naturales Renovables. Grupo de Investigación en Sanidad Acuícola, Inocuidad y Salud Ambiental. Facultad de Acuicultura y Ciencias del Mar. Universidad Técnica de Manabí Bahía de Caráquez, Manabí EC130104, Ecuador. https://orcid.org/0000-0002-6660-6940

DOI:

https://doi.org/10.33936/at.v5i3.5980

Keywords:

Aquaculture, Bacillus, shrimp, freshwater culture, yeasts, potassium

Abstract

Probiotics are commonly used in aquaculture for their benefits for growth, disease prevention and stress reduction. However, the benefits of probiotics in shrimps grown at low salinity have been little studied even though this type of aquaculture has increased considerably in the last decade. In this sense, this study aims to evaluate the effects of probiotics on the survival, increase in weight and length, and total protein concentration in hemolymph of Penaeus vannamei faced with ionic imbalance. A completely randomized experimental design was applied with 288 juveniles of P. vannamei (9.80 ± 1.33 g; 12.11 ± 1.06 cm) distributed in 24 aquariums of 40 L. A control at 30 ‰ and three treatments at 2 ‰ (n = 8) with three replicates each was used. All groups were duplicated, and probiotics (Aquablend C® or HLBAC®) were added to one of the duplicates. To generate the ionic imbalance, magnesium was increased in one of the 2 ‰ treatments and potassium in the other. The bioassay lasted 96 h, and the shrimp were fed with a ration equivalent to 4% of biomass every 8 h. Water quality parameters, initial and final length and weight, daily mortality and total proteins in hemolymph were recorded, to compare among treatments. Survival was significantly reduced with ion imbalance; in the treatments with ion modification, survival was significantly greater when probiotics were incorporated. The increase in length and weight in treatments at low salinity was significantly lower than in treatments at 30 ‰. The total protein concentration in hemolymph did not vary significantly among treatments. The use of probiotics significantly improved the survival of P. vannamei against ionic imbalance at 2 ‰, which opens a new line of research for the development of low salinity shrimp culture protocols.

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