Efectos de la suplementación dietética con Spirulina subsalsa sobre el crecimiento y la pigmentación en Betta splendens
Effects of dietary Spirulina subsalsa supplementation on growth performance and pigmentation in Betta splendens
DOI:
https://doi.org/10.33936/at.v8i2.8444Keywords:
cyanobacteria , diet , ornamental fish , pigmentation , performance , growthAbstract
Cyanobacteria of the genus Spirulina contain high levels of protein, carotenoids, and phycocyanin, compounds associated with improved growth and pigmentation in ornamental fish. This study evaluated the effect of dietary inclusion of Spirulina subsalsa on the growth performance and pigmentation of Betta splendens. A completely randomized experimental design was used with five treatments: a control diet without cyanobacteria and diets containing Spirulina subsalsa at 5, 10, 15, and 18%. Each treatment was evaluated in triplicate using 2 L experimental units over a five-week period. Fish length and weight were measured weekly, and weight gain (WG), specific growth rate (SGR), and condition factor (K) were calculated. Fish showed growth in all treatments; however, no significant differences were detected among treatments in length, weight, WG, SGR, or K (P > 0.05). The highest final weight was observed in the 18% inclusion treatment (0.49 g), whereas the greatest mean lengths were recorded in the 10% (3.38 cm) and 18% (3.37 cm) treatments, with no significant differences compared with the control. No changes in fish pigmentation were observed among treatments. These results indicate that Spirulina subsalsa may serve as a dietary supplement but not as a complete diet for Betta splendens, and that pigmentation was primarily associated with genetic factors.
Downloads
References
Adel M., Yeganeh S., Dadar M., Sakai M., Dawood M.A.O. (2016). Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754). Fish & Shellfish Immunology 56:436–444. https://doi.org/10.1016/j.fsi.2016.08.003
Bennet A., Bogorad L. 1973. Complementary chromatic adaptation in filamentous blue-green alga. Journal of Cell Biology 58:419-435. https://doi.org/10.1083/jcb.58.2.419
Borrego-Enriquez L.E., Muñoz F.G. (2013). Identificación de componentes de las burbujas protectoras producidas por el macho del pez Betta (Betta splendens). Investigación Universitaria Multidisciplinaria (12):145-149.
Cisneros-Morales J.A. (2019). Evaluación del efecto del medio de cultivo en la composición química de los pigmentos de Arthrospira maxima Setchell & N.L. Gardner in N.L. Gardner 1917. Tesis de Licenciatura en Biología Marina, Universidad del Mar, campus Puerto Ángel, Puerto Ángel, Oaxaca, México.
Dar B.A., Khaliq R., Jha G.N., Kour P., Qureshi T.A. (2014). Protective effects of dietary Spirulina against cadmium chloride exposed histoarchitectural changes in the liver of freshwater catfish Clarias batrachus (Linnaeus, 1758). Indian Journal of Fisheries 61(3):83–87.
Dernekbasi S., Unal H., Karayucel I., Aral O. (2010). Effect of dietary supplementation of different rates of Spirulina (Spirulina platensis) on growth and feed conversion in Guppy (Poecilia reticulata Peters, 1860). Journal of Animal and Veterinary Advances 9(9):1395–1399.
Díaz‐Jiménez L., Hernández‐Vergara M.P., Pérez‐Rostro C.I., Olvera‐Novoa M.Á. (2021). The effect of two carotenoid sources, background colour and light spectrum on the body pigmentation of the clownfish Amphiprion ocellaris. Aquaculture Research 52(7):3052-3061. https://doi.org/10.1111/are.15149
Díaz-Jiménez L., Hernández-Vergara M.P., Pérez-Rostro C.I., Ortega-Clemente L.A. (2019). The effect of astaxanthin and β-carotene inclusion in diets for growth, reproduction and pigmentation of the peppermint shrimp Lysmata wurdemanni. Latin American Journal of Aquatic Research 47(3):559-567. http://dx.doi.org/10.3856/vol47-issue3-fulltext-17
Ezquerra-Brauer J.F., Chan-Higuera J.E. (2021). Capacidad antioxidante y mecanismo de acción de pigmentos en organismos marinos. Ciencia UAT. 15(2):186-197. https://doi.org/10.29059/cienciauat.v15i2.1501
James R., Sampath K., Nagarajan R., Vellaisamy P., Manikandan M.M. (2009). Effect of dietary Spirulina on reduction of copper toxicity and improvement of growth, blood parameters and phosphatases activities in carp, Cirrhinus mrigala (Hamilton, 1822). Indian Journal of Experimental Biology 47(9):754–759.
Jeffrey S.W., Humphrey G.F. 1975. New specthophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural populations. Biochimie and Phisiology Pflanze 167:191-194.
Hancz C., Magyary I., Molnár T., Sato S., Horn P., Taniguchi N. (2003). Evaluation of color intensity enhanced by paprika as feed additive in goldfish and koi carp using computer-assisted image analysis. Fisheries Science 69:1158-1161. https://doi.org/10.1111/j.0919-9268.2003.00740.x
Humphrey G.F. 1979. Photosynthetic characteristics of algae grow under constant illumination and light dark regimes. Journal of Experimental Marine Biology and Ecology 40:63-70. https://doi.org/10.1016/0022-0981(79)90034-0
Kim S.S., Shin S.J., Han H.S., Kim J.D., Lee K.J. (2015) Effects of dietary Spirulina pacifica on innate immunity and disease resistance against Edwardsiella tarda in olive flounder Paralichthys olivaceus. The Israeli Journal of Aquaculture 67:9. http://hdl.handle.net/10524/49181
Kiswara C.A., Budiharjo A., Sari S.L.A. (2020). Changes in color of betta fish (Betta splendens) by feeding of Artemia salina enriched with Tagetes erecta flower flour. Cell Biology and Development 4(2):46–50. https://doi.org/10.13057/cellbioldev/v040202
Komilus C.F., Mohamad-Zuki N.A., Aminudin N.H., Redhwan A.I., Mohd-Khairulnizam N.A.N. (2023). Effects of crab shell waste as feed on growth performance and colouration of Siamese fighting fish (Betta splendens). Malaysian Applied Biology 52(5):213–220. https://doi.org/10.55230/mabjournal.v52i5.fisas10
Kumar, D., Dhar, D. W., Pabbi, S., Kumar, N., & Walia, S. (2014). Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540). Indian Journal of plant physiology, 19(2):184-188.
Maza L.R., Guevara M., Bernal J.F. (2018). Crecimiento y pigmentos de la Spirulina subsalsa cultivada a diferentes salinidades y concentraciones de nitrógeno. MUTIS, 8(2):25-36. http://orcid.org/0000-0003-1072-6596
Norazmi-Lokman N.H., Baderi A.A., Zabidi Z.M., Diana A.W. (2020). Effects of different feeding frequency on Siamese fighting fish (Betta splendens) and guppy (Poecilia reticulata) juveniles: Data on growth performance and survival rate. Data in Brief 32:106046. https://doi.org/10.1016/j.dib.2020.106046
Patria M.P., Amanda S.P., Susanti H., Susilaningsih D., Taufikurahman T. (2024). Growth response and color brightness of betta fish (Betta splendens) supplemented by Spirulina powder from Arthrospira maxima. Journal of Agricultural Science and Technology, 26(1):76-83. https://doi.org/10.22034/JAST.26.1.73
Rahimnejad S. (2013) Partial replacement of fish meal with Spirulina pacifica in diets for Parrot Fish (Oplegnathus fasciatus). Turkish Journal of Fisheries & Aquatic Sciences, 13 (13):197–204. https://doi.org/10.4194/1303-2712-v13_2_01
Rincón D.D., Velásquez H.A., Dávila M.J., Semprún A.M., Morales E.D., Hernández J.L. (2012). Substitution levels of fish meal by Arthrospira (=Spirulina) maxima meal in experimental diets for Red Tilapia Fingerlings (Oreochromis sp.). Revista Colombiana de Ciencias Pecuarias 25:430–437.
Rojas-Villalta D., Gómez-Espinoza O., Guillén-Watson R., Murillo-Vega F., Villalta-Romero F., Vaquerano-Pineda F., Chicas M.,
Guerrero M., Núñez-Montero K. (2024). Impact of Arthrospira maxima feed supplementation on gut microbiota and growth performance of Tilapia Fry (Oreochromis niloticus). Fishes, 9(10):374. https://doi.org/10.3390/fishes9100374
Saekhow S., Nuntapong N., Rungruangsak-Torrissen K., Thongprajukaew K. (2022). Efficiency of homemade egg-based diet for male Siamese fighting fish (Betta splendens). Science Asia 48:664-672. https://doi.org/10.2306/scienceasia1513-1874.2022.096
Sadat-Ghoreyshi T., Naeimpoor F. (2025). Tailored illumination augmenting grow and phycocyanin production by Spirulina platensis: a mixture design approach. Algal Research 90(1):1–12. https://doi.org/10.1016/j.algal.2025.104129
Susanti J.E., Singgih M., Mulyani L.N. (2019). Optimization of extraction method and characterization of phycocyanin pigment from Spirulina platensis. Journal of Mathematical and Fundamental Sciences 51(2):168-176. https://doi.org/10.5614/j.math.fund.sci.2019.51.2.6
Torres-Ariño A., Hernández de Dios M.A., Carrasco-López G. (2021). Efecto de la irradiancia en el crecimiento y coloración de la cianobacteria marina Spirulina subsalsa Oersted ex Gomont, 1892. AquaTechnica, 3(1):25-36. https://doi.org/10.33936/at.v3i1.3483
Torres-Ariño, A. (2019). Manual de técnicas básicas para el estudio de cianobacterias y microalgas. Curso Biotecnología de cianobacterias y microalgas: aislamiento, cultivo y aplicación. Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas y Universidad del Mar, campus Puerto Ángel. Monterrey, Nuevo León, México. 71 pp.
Vasudhevan I., James R. (2011) Effect of optimum Spirulina along with different levels of vitamin C incorporated diets on growth, reproduction and coloration in goldfish Carassius auratus (Linnaeus, 1758). Indian Journal of Fisheries 58(2):101–106.
Vivaldo-Patraca L.G. (2019). Crecimiento de caracol Manzana Pomacea bridgesii (Reeve, 1856) utilizando dos dietas artificiales elaboradas: con y sin adición de Spirulina subsalsa Oersted ex Gomont 1892. Tesis de Licenciatura en Ingeniería en Acuicultura, Universidad del Mar, campus Puerto Ángel, Oaxaca, México.
Vu T.D., Iwasaki Y. (2020). Behavioral and brain-transcription synchronization between the two opponents of fighting pair of the Betta splendens. PLOS Genetics, 16(6):e1008831. https://doi.org/10.1371/journal.pgen.1008831
Yuslan A., Nasir N., Suhaimi H., Arshad A., Rasdi N.W. (2021). The effect of enriched cyclopoid copepods on the coloration and feeding rate of Betta splendens. IOP Conference Series: Earth and Environmental Science 869:012007. https://doi.org/10.1088/1755-1315/869/1/012007
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Cristopher Giovanni Renero-Mondragón, Marco Antonio Hernández-de Dios, Alfredo Gallardo-Collí, Alejandra Torres Ariño

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
