Antagonistic activity of bacteria isolated from marine ecosystems against vibrio parahaemolyticusAHPND as a pathogen of aquaculture importance

Abstract

Regards the aquaculture sector, one of the largest sectors of food production is shrimp farming, however, it has been affected by diseases, including Acute Hepatopancreas Necrosis Disease (AHPND). which is caused by Vibrio parahaemolyticus, which host a plasmid who produce toxins PirA and Pir B. An alternative method to control the pathogen is the application of probiotics, when consumed by the shrimp, give it a defense mechanism against pathogens. In this investigation marine ecosystems were evaluated and marine bacteria were isolated which, with the well diffusion test, showed an antagonistic effect against the pathogenic shrimp strain, where inhibition halos were observed from 1cm to almost 4 cm in diameter; In addition, these bacteria were characterized by a biochemical profile with the BioMérieux API system and by sequencing the 16S rDNA gene where the genera Shewanella sp., Vibrio sp. and Bacillus sp. The ability of these bacteria to be potentially used as a prophylactic and / or therapeutic measure in shrimp farms is of paramount importance, and avoid the use of antibiotics.