Spat nursery of the American oyster Crassostrea virginica with application of probiotic actinomycetes and homeopathic medicines
DOI:
https://doi.org/10.33936/at.v2i3.3086Keywords:
Aquacultural Homeopathy, Probiotic actinomycetes, Crassostrea virginica, Physiological conditionAbstract
The effect of probiotic actinobacteria and homeopathic medicines, as well as their combination, on the physiological condition of early juveniles of American oyster Crassostrea virginica during their nursing in a commercial oyster laboratory was evaluated for 30 days. Five treatments were included in the experimental design: [T1 (Streptomyces sp. RL8), T2 (ViP 7C + VIA 7C), T3 (PhA 7C + SIT 7C), T4 (ViP 7C + VIA 7C + RL8), T5 (PhA 7C + SIT 7C + RL8)] and two controls: [T6 (Ethanol) and T7 (Distilled Water)], with three replicates each. As physiological response variables, the Digestive Gland Index (IGD) and the Muscular Index (MI) were evaluated. At the end of the bioassay (T30), a higher average value of the IGD was observed in juveniles that received the treatments T3, T4 and T5, as well as differences in relation to the MI, with maximum values in juveniles that received T3, T5, and T6. These results demonstrate that the use of probiotic actinomycetes and homeopathic medicines has productive applicability to improve the physiological condition and performance in C. virginica, with a positive potential effect on their biological and economic performance during hatchery-nursery stage and sea farming.
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References
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García-Bernal M., Medina-Marrero R., Campa-Córdova Á.I., Mazón-Suástegui, J.M., (2017). Probiotic effect of Streptomyces strains alone or in combination with Bacillus and Lactobacillus in juveniles of the white shrimp Litopenaeus vannamei. Aquaculture International, 25:927-939.
García-Bernal M., Medina-Marrero R., Rodríguez-Jaramillo C., Marrero-Chang O., Campa-Córdova Á. I., Medina-García R., Mazón-Suástegui J.M., (2018). Probiotic effect of Streptomyces spp. on shrimp (Litopenaeus vannamei) postlarvae challenged with Vibrio parahaemolyticus. Aquaculture Nutrition, 24:865-871.
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García-Bernal M., Medina-Marrero R., Campa-Córdova Á.I., Tovar-Ramírez D., Barajas D., Ormart-Castro P., Mazón-Suástegui J.M., (2020). Growth and survival of the shrimp Penaeus vannamei with application of probiotic actinomycetes and homeopathy. AquaTechnica, 2:76-85.
Hasan K.N., Banerjee, G., (2020). Recent studies on probiotics as beneficial mediator in aquaculture: a review. The Journal of Basic and Applied Zoology. 81:1-16.
Immanuel G., Citarasu T.,Sivaram V., Michael Babu M., Palavesam A., (2007). Delivery of HUFA, probionts and biomedicine through bioencapsulated Artemia as a means to enhance the growth and survival and reduce the pathogenicity in shrimp Penaeus monodon postlarvae. Aquaculture International. 15:137–152.
Irianto A., Austin B., (2002). Probiotics in Aquaculture. Journal of Fish Diseases. 25:633–642.
Kuebutornye F.K.A., Abarike E.D., Lu Y., (2019). A review on the application of Bacillus in aquaculture. Fish & Shellfish Immunology. 87:820-828.
Kiarazm M., Tajik P., Nava H.G., (2011). Assessment of the effect of homoeopathic nosodes in subclinical bovine mastitis. Annals of biological research. 2:552-562.
Lalloo R., Moonsamy G., Ramchuran S., Görgens J., Gardiner, N., (2010). Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent. Letters in Applied Microbiology. 50:563–570.
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Mazón-Suástegui J.M., García-Bernal M., Avilés-Quevedo A., Campa-Córdova A.I., Salas-Leiva J., Abasolo-Pacheco F., (2018a). Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei. Rev. MVZ Córdoba, 23:6850-6859.
Mazón-Suástegui, J.M., Tovar-Ramírez, D., Salas-Leiva, J. S., Arcos-Ortega, G.F., García-Bernal, M., Avilés-Quevedo, M.A., López-Carvallo, J.A., García-Corona, J.L., Ibarra-García, L.E., Ortíz-Cornejo, N.L., Teles, A., Rosero-García, A., Abasolo-Pacheco, F., Campa-Cordova, A.I., Saucedo Lastra, P.E., Barajas Frias, J.D., Ormart Castro, P., Rodriguez Jaramillo, M.C., González-González, R., Barajas-Ponce, U., Tordecillas-Guillén, J.L., Álvarez-Gil, F.A., Pineda-Mahr, G., Peiro-López, J., Robles-Mungaray, M., (2018b). Aquacultural Homoeopathy: A Focus on Marine Species. In: Diarte-Plata G and Escamilla R (eds), “Aquaculture: Plants and Invertebrates”. IntechOpen Books. DOI: http://dx.doi.org/19.5772/intechopen.78030.
Mazón-Suástegui J.M., Leyva-Miranda G.A., Arrieche-Galíndez D., Lodeiros-Seijo C., López-Carvallo J.A., (2019a). Influence of hatchery rich-carbohydrates diet on the oyster Crassostrea corteziensis (Hertlein, 1951). Aquaculture Research.. 50(10):3078-3081.
Mazón-Suástegui J. M., Ojeda-Silvera C. M., García-Bernal M., Avilés-Quevedo M. A., Abasolo-Pacheco F., Batista-Sánchez D., Tovar-Ramírez D., Arcos-Ortega F., Murillo-Amador B., Nieto-Garibay A., Ferrer-Sánchez Y., Morelos-Castro R. M., Alvarado-Mendoza A., Díaz-Díaz M., & Bonilla-Montalvan B., (2019b). Agricultural homeopathy: New insights into organicʼs. In: Jan Moudry (ed.) “Multifunctionality and Impacts of Organic Agriculture”. IntechOpen Books. DOI: http://dx.doi.org/19.5772/intechopen.84482.
Mazón-Suástegui J.M., Salas-Leiva J., Teles A., Tovar-Ramírez D., (2019c). Immune and antioxidant enzyme response of Longfin yellowtail (Seriola rivoliana) juveniles to ultra-diluted substances derived from phosphorus, silica and pathogenic Vibrio. Homeopathy, 108:43-53.
Shah R., (2014). Scientific method of preparing homoeopathic nosodes. Indian Journal of Research in Homoeopathy. 8:166–173.
Shinn A.P., Pratoomyot J., Griffiths D., Trong T.Q., Vu, N.T., Jiravanichpaisal P., Briggs M., (2018). Asian shrimp production and the economic costs of disease. Asian Fisheries Science, 31S:30-58.
Sugita H., Matsuo N., Hirose Y., Iwato M., Deguchi, Y., (1997). Vibrio sp. strain NM 10, isolated from the intestine of a Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida. Applied and Environmental Microbiology. 63:4986–4989.
Sugita H., Ohta K., Kuruma A., Sagesaka, T., (2007). An antibacterial effect of Lactococcus lactis isolated from the intestinal tract of the Amur catfish, Silurus asotus Linnaeus. Aquaculture research. 38:1002–1004.
Valentim-Zabott M., Vargas, L., Ribeiro, R. P. R., Piau, R., Torres, M. B. A., Rönnau, M., Souza, J. C., (2008). Effects of a homeopathic complex in Nile tilapia (Oreochromis niloticus L.) on performance, sexual proportion and histology. Homeopathy, 97:190-195.
Verschuere L., G. Rombaut P. Sorgeloos, Verstraete W., (2000). Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews. 64:655–671.
Zorriehzahra M.J., Delshad S.T., Adel M., Tiwari R., Karthik K., Dhama D., Lazado C.C., (2016). Probiotics as beneficial microbes in aquaculture: an update on their multiple modes of action: a review. Veterinary Quaterly, 36:228-241.
Balcazar J.L., Blas I., Ruiz-Zarzuela I., Cunningham, D., Vendrell D., Muzquiz J.L., (2006). The role of probiotics in aquaculture. Veterinary Microbiology. 114:173–186.
Desriac F., Defer D., Bourgougnon N., Brillet B., Le Chevalier P., Fleury, Y., (2010). Bacteriocin as weapons in the marine animal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic. Marine drugs. 8:1153–1177.
FAO, (2020). El estado mundial de la pesca y la acuicultura 2020. La sostenibilidad en acción. Roma. https://doi.org/10.4060/ca9229es.
Gabbott, P., (1983). Developmental and seasonal metabolic activities in marine mollusks. In: P.W. Hochachka (ed.). The Mollusca 2: environmental biochemistry and physiology, Academic Press, New York, pp. 165-217.
García-Bernal M., Campa-Córdova A.I., Saucedo P.E., Casanova-González M., Medina-Marrero R., Mazón-Suástegui J.M., (2015). Isolation and in vitro selection of actinomycetes strains as potential probiotics for aquaculture. Veterinary World, 8:170-176.
García-Bernal M., Medina-Marrero R., Campa-Córdova Á.I., Mazón-Suástegui, J.M., (2017). Probiotic effect of Streptomyces strains alone or in combination with Bacillus and Lactobacillus in juveniles of the white shrimp Litopenaeus vannamei. Aquaculture International, 25:927-939.
García-Bernal M., Medina-Marrero R., Rodríguez-Jaramillo C., Marrero-Chang O., Campa-Córdova Á. I., Medina-García R., Mazón-Suástegui J.M., (2018). Probiotic effect of Streptomyces spp. on shrimp (Litopenaeus vannamei) postlarvae challenged with Vibrio parahaemolyticus. Aquaculture Nutrition, 24:865-871.
García-Bernal M., Medina-Marrero R., Campa-Córdova Á.I., Mazón-Suástegui J.M., (2019). Growth and antioxidant response of juvenile oysters Crassostrea sikamea and Crassostrea corteziensis treated with Streptomyces strains. Arquivo Brasileiro de Medicina Veterinaria y Zootecnia, 71:1993-1998.
García-Bernal M., Medina-Marrero R., Campa-Córdova Á.I., Tovar-Ramírez D., Barajas D., Ormart-Castro P., Mazón-Suástegui J.M., (2020). Growth and survival of the shrimp Penaeus vannamei with application of probiotic actinomycetes and homeopathy. AquaTechnica, 2:76-85.
Hasan K.N., Banerjee, G., (2020). Recent studies on probiotics as beneficial mediator in aquaculture: a review. The Journal of Basic and Applied Zoology. 81:1-16.
Immanuel G., Citarasu T.,Sivaram V., Michael Babu M., Palavesam A., (2007). Delivery of HUFA, probionts and biomedicine through bioencapsulated Artemia as a means to enhance the growth and survival and reduce the pathogenicity in shrimp Penaeus monodon postlarvae. Aquaculture International. 15:137–152.
Irianto A., Austin B., (2002). Probiotics in Aquaculture. Journal of Fish Diseases. 25:633–642.
Kuebutornye F.K.A., Abarike E.D., Lu Y., (2019). A review on the application of Bacillus in aquaculture. Fish & Shellfish Immunology. 87:820-828.
Kiarazm M., Tajik P., Nava H.G., (2011). Assessment of the effect of homoeopathic nosodes in subclinical bovine mastitis. Annals of biological research. 2:552-562.
Lalloo R., Moonsamy G., Ramchuran S., Görgens J., Gardiner, N., (2010). Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent. Letters in Applied Microbiology. 50:563–570.
López-Carvallo J.A., Arcos-Ortega G. F., Tovar-Ramírez D., Hernández-Oñate M.Á., Abasolo-Pacheco F., García-Corona J.L., Mazón-Suástegui J.M., (2019) Effect of immunomodulatory medication over the general response of juvenile Catarina scallop (Argopecten ventricosus Sowerby II, 1842). Latin American Journal of Aquatic Research, 47:65-77.
Mazón-Suástegui J.M., Rosero-García A., Avilés- Quevedo A., Dumas S., Vega R., Rodríguez- Jaramillo C., Tovar-Ramírez D., (2016). Homeopathy for marine fish aquaculture: Increased growth and survival of juvenile spotted rose snapper Lutjanus guttatus. Homeopathy, 105:32- 33.
Mazón-Suástegui J.M., García-Bernal M. Saucedo P.E., Campa-Córdova A.I., Abasolo-Pacheco F., (2017). Homeopathy outperforms antibiotics in juvenile scallop Argopecten ventricosus: Effects on growth, survival, and immune response. Homeopathy, 106: 18-26.
Mazón-Suástegui J.M., García-Bernal M., Avilés-Quevedo A., Campa-Córdova A.I., Salas-Leiva J., Abasolo-Pacheco F., (2018a). Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei. Rev. MVZ Córdoba, 23:6850-6859.
Mazón-Suástegui, J.M., Tovar-Ramírez, D., Salas-Leiva, J. S., Arcos-Ortega, G.F., García-Bernal, M., Avilés-Quevedo, M.A., López-Carvallo, J.A., García-Corona, J.L., Ibarra-García, L.E., Ortíz-Cornejo, N.L., Teles, A., Rosero-García, A., Abasolo-Pacheco, F., Campa-Cordova, A.I., Saucedo Lastra, P.E., Barajas Frias, J.D., Ormart Castro, P., Rodriguez Jaramillo, M.C., González-González, R., Barajas-Ponce, U., Tordecillas-Guillén, J.L., Álvarez-Gil, F.A., Pineda-Mahr, G., Peiro-López, J., Robles-Mungaray, M., (2018b). Aquacultural Homoeopathy: A Focus on Marine Species. In: Diarte-Plata G and Escamilla R (eds), “Aquaculture: Plants and Invertebrates”. IntechOpen Books. DOI: http://dx.doi.org/19.5772/intechopen.78030.
Mazón-Suástegui J.M., Leyva-Miranda G.A., Arrieche-Galíndez D., Lodeiros-Seijo C., López-Carvallo J.A., (2019a). Influence of hatchery rich-carbohydrates diet on the oyster Crassostrea corteziensis (Hertlein, 1951). Aquaculture Research.. 50(10):3078-3081.
Mazón-Suástegui J. M., Ojeda-Silvera C. M., García-Bernal M., Avilés-Quevedo M. A., Abasolo-Pacheco F., Batista-Sánchez D., Tovar-Ramírez D., Arcos-Ortega F., Murillo-Amador B., Nieto-Garibay A., Ferrer-Sánchez Y., Morelos-Castro R. M., Alvarado-Mendoza A., Díaz-Díaz M., & Bonilla-Montalvan B., (2019b). Agricultural homeopathy: New insights into organicʼs. In: Jan Moudry (ed.) “Multifunctionality and Impacts of Organic Agriculture”. IntechOpen Books. DOI: http://dx.doi.org/19.5772/intechopen.84482.
Mazón-Suástegui J.M., Salas-Leiva J., Teles A., Tovar-Ramírez D., (2019c). Immune and antioxidant enzyme response of Longfin yellowtail (Seriola rivoliana) juveniles to ultra-diluted substances derived from phosphorus, silica and pathogenic Vibrio. Homeopathy, 108:43-53.
Shah R., (2014). Scientific method of preparing homoeopathic nosodes. Indian Journal of Research in Homoeopathy. 8:166–173.
Shinn A.P., Pratoomyot J., Griffiths D., Trong T.Q., Vu, N.T., Jiravanichpaisal P., Briggs M., (2018). Asian shrimp production and the economic costs of disease. Asian Fisheries Science, 31S:30-58.
Sugita H., Matsuo N., Hirose Y., Iwato M., Deguchi, Y., (1997). Vibrio sp. strain NM 10, isolated from the intestine of a Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida. Applied and Environmental Microbiology. 63:4986–4989.
Sugita H., Ohta K., Kuruma A., Sagesaka, T., (2007). An antibacterial effect of Lactococcus lactis isolated from the intestinal tract of the Amur catfish, Silurus asotus Linnaeus. Aquaculture research. 38:1002–1004.
Valentim-Zabott M., Vargas, L., Ribeiro, R. P. R., Piau, R., Torres, M. B. A., Rönnau, M., Souza, J. C., (2008). Effects of a homeopathic complex in Nile tilapia (Oreochromis niloticus L.) on performance, sexual proportion and histology. Homeopathy, 97:190-195.
Verschuere L., G. Rombaut P. Sorgeloos, Verstraete W., (2000). Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews. 64:655–671.
Zorriehzahra M.J., Delshad S.T., Adel M., Tiwari R., Karthik K., Dhama D., Lazado C.C., (2016). Probiotics as beneficial microbes in aquaculture: an update on their multiple modes of action: a review. Veterinary Quaterly, 36:228-241.
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2020-12-31
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